首页> 外文期刊>BioTechnology: An Indian Journal >Quick separation and determination of 6 lignans in Schisandra Chinensis (Turcz.) Baill and Schisand rasphenanthera Rehd. Et Wils using capillaryelectrophoresis
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Quick separation and determination of 6 lignans in Schisandra Chinensis (Turcz.) Baill and Schisand rasphenanthera Rehd. Et Wils using capillaryelectrophoresis

机译:快速分离和测定五味子中的6种木脂素和五味子。 Et Wils使用毛细管电泳

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摘要

To set up a method for quick determination of 6 lignans in Schisandra Chinensis using capillary electrophoresis. The paper discussed the influence of factors, such as buffer solution, additives, separation voltage, temperature and sample-loading conditions, on the separation and determination of lignans. The Schisandra chinensis sample solution can be analyzed under the optimal conditions of 10mmol/L NaH2PO4-Na2HPO4 buffer solution (35% acetonitrile, 37.5mmol/L SDS at pH 8) and separation voltage 28.0KV. The linear range is 25.5~1020, 25.75~1030, 12~480, 25.5~1020, 24.25~970 and 19.75~790 mg/L respectively; the detection limit is 0.5, 0.8, 1.0, 1.0, 1.0 and 1.5 mg/L. By comparing to the high performance liquid chromatography, the present method has remarkable advantages in the separation effect, column efficiency and analysis speed.
机译:建立毛细管电泳快速测定五味子中6种木脂素的方法。本文讨论了缓冲液,添加剂,分离电压,温度和样品加载条件等因素对木脂素分离和测定的影响。五味子样品溶液可以在10mmol / L NaH2PO4-Na2HPO4缓冲溶液(35%乙腈,37.5mmol / L SDS,pH 8)和分离电压28.0KV的最佳条件下进行分析。线性范围分别为25.5〜1020、25.75〜1030、12〜480、25.5〜1020、24.25〜970和19.75〜790 mg / L;检出限为0.5、0.8、1.0、1.0、1.0和1.5 mg / L。与高效液相色谱法相比,本方法在分离效果,柱效和分析速度上具有明显的优势。

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