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首页> 外文期刊>BioTechnology: An Indian Journal >Genetic variability in accession of Lathyrus inconspicuous based on protein analysis on SDS-PAGE
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Genetic variability in accession of Lathyrus inconspicuous based on protein analysis on SDS-PAGE

机译：基于SDS-PAGE蛋白质分析的山thy豆的遗传变异不明显

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Eighteen L. inconspicuous accessions were evaluated for variations in 100 seeds weight, seed proteins content, and electrophoretic patterns of the seed proteins. The 100 seeds weight and seed proteins content ranged between 0.885 g to 1.995 g and 147mg/g seedmeal to 109 mg/g seedmeal respectively, exhibiting a reasonable genetic diversity for these traits. The variation between the seed size of these accessions was attributed to the development process and the environmental condition to which the mother plant is exposed, whereas the variation in total seed proteins content may be due to genotype and/or seasonal influences. Interestingly, there is no correlation between seed proteins content and 100 seeds weight indicating that the two traits are genetically independent. The variation in the electrophoretic pattern located in the regions of the gel contain the bands with molecular weight more than 98 KD, heavy subunits of alphalathyrin subunits and bands with molecular weight around 70KD. Multivariate analysis of SDS-PAGE data showed reasonable genetic variability among the accessions and a low variability among the accessions of the same region. It also showed that the accessions collected from Turkey were distributed between more than on cluster, indicating relatively high variation in the genetic diversity of these accessions. Moreover, it revealed there is no relation between genetic diversity and geographic distribution. The separation of all accessions on the first principal coordinate analysis indicated a good association between accessions which was probably attributed to parallel evolution. Based on genetic diversity between these accessions, improvement through simple selection for these traits is possible.
机译：评价了18个不显眼的L.种质的100个种子重量，种子蛋白含量和种子蛋白的电泳模式的变化。 100粒种子的重量和种子蛋白质含量分别在0.885 g至1.995 g和147mg / g种子粉至109 mg / g种子粉之间，表现出这些特征的合理遗传多样性。这些种质的种子大小之间的差异归因于发育过程和母本植物所处的环境条件，而总种子蛋白含量的差异可能归因于基因型和/或季节影响。有趣的是，种子蛋白质含量和100种子重量之间没有相关性，表明这两个性状在遗传上是独立的。位于凝胶区域的电泳图谱的变化包含分子量大于98 KD的条带，αlathyrin亚基的重亚基和分子量大约70 KD的条带。 SDS-PAGE数据的多变量分析表明，同一地区的种质之间合理的遗传变异性和种质之间的低变异性。研究还表明，从土耳其收集的种质分布在多于簇系之间，表明这些种质的遗传多样性差异较大。而且，它揭示了遗传多样性和地理分布之间没有关系。第一次主坐标分析中所有种质的分离表明种质之间的良好关联，这可能归因于平行进化。基于这些种质之间的遗传多样性，可以通过简单选择这些性状来进行改良。

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