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首页> 外文期刊>BioTechnology: An Indian Journal >Molecular characterization of cholera autoinducer-1(CAI-1) mimic as a potent cqsS receptor agonist
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Molecular characterization of cholera autoinducer-1(CAI-1) mimic as a potent cqsS receptor agonist

机译：霍乱自诱导物1（CAI-1）模拟作为强效cqsS受体激动剂的分子表征

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摘要

The binding of the signaling molecule CholeraAuto inducer-1 (CAI-1) to the cognate transmembrane receptor CqsS regulates the behavioural pattern in Vibrio cholerae. CAI-1 serves as the liaison between Vibrio species thereby, regulating their virulence & pathogenecity. This study highlights on the in-vitro characterization of CAI-1 mimicmolecules from Melia dubia leaf extract. The methanol fraction of the aqueous leaf extract thatmuffled theHaemolyis&Biofilmformation simultaneously, enhancing the production of extracellular protease and oxidative stress response in a Low Cell Density (LCD) condition was subjected to GC-MS analysis which unmasked an array of 19 compounds. Molecular Docking was chosen as a tool to predict the compound that had a greater binding affinity to CqsS receptor than the native CAI-1. Consequently, through in-silico analysis it was found out that 4-Ethyl Resorcinol in the methanol fraction had the maximum binding affinity. Further experiments in-vitro, validated the prediction that 4-Ethyl Resorcinol in Melia dubia leaves was a potent CAI-1 functionalmimic molecule i.e. CqsS receptor agonist.
机译：信号分子CholeraAuto Inducer-1（CAI-1）与同源跨膜受体CqsS的结合调节了霍乱弧菌的行为模式。 CAI-1可作为弧菌之间的联络人，从而调节其毒力和致病性。这项研究着重介绍了来自Melia dubia叶子提取物的CAI-1模拟分子的体外表征。对水提取物的甲醇级分同时掩盖了血红蛋白和生物膜的形成，从而在低细胞密度（LCD）条件下增强了细胞外蛋白酶的产生和氧化应激反应，并对其进行了GC-MS分析，发现了19种化合物。选择分子对接作为预测与天然CAI-1相比具有更大的CqsS受体结合亲和力的化合物的工具。因此，通过计算机内分析发现，甲醇级分中的4-乙基间苯二酚具有最大的结合亲和力。进一步的体外实验验证了Mel叶中4-乙基间苯二酚是有效的CAI-1功能模拟分子即CqsS受体激动剂的预测。

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