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首页> 外文期刊>BioTechnology: An Indian Journal >Research on phytoestrogenic effective fraction of cuscuta chinensis
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Research on phytoestrogenic effective fraction of cuscuta chinensis

机译：中华cut植物雌激素有效成分的研究

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Effective fraction of Cuscuta chinensis were studied using a combination of in vitro and in vivo assays. Estrogen-like activity was determined by uterus growth test in low and high estrogen female model mice. Then MTT assay of the MCF-7 cells was conducted with the medicated serum of the mice. Estrogen-antagonistic effect was also determined by uterus rate of mice. 95%ethanol extracts of Cuscuta chinensis dose of 24 g ??? kg- 1 ??? d-1 after intragastric administration increased the uterus rate of immature mice; The medicated serum of 24 g ??? kg-1 ??? d-1 significantly promoted the proliferation of MCF-7 cells. 24 g ??? kg-1 ??? d-1 95% ethanol extracts + diethylstilbestrol significantly inhibited the growth of the uterus of high estrogen model mice. 95% ethanol extracts were effective fraction of Cuscuta chinensis. The method established is accurate, reliable, which can be used for the further studies on the phytoestrogen material basis of Cuscuta chinensis.
机译：使用体外和体内试验相结合的方法研究了中华Cu的有效成分。通过子宫生长试验在低和高雌激素雌性模型小鼠中确定雌激素样活性。然后用小鼠的含药血清对MCF-7细胞进行MTT测定。雌激素拮抗作用也通过小鼠子宫率来确定。中华ethanol的95％乙醇提取物剂量为24克公斤-1 ???胃内给药后d-1增加未成熟小鼠的子宫率; 24克药血清公斤1 ??? d-1显着促进MCF-7细胞的增殖。 24克???公斤1 ??? d-1 95％乙醇提取物+己烯雌酚显着抑制高雌激素模型小鼠子宫的生长。 95％的乙醇提取物是中华Cu的有效成分。建立的方法准确，可靠，可用于中华for的植物雌激素物质基础的进一步研究。

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