• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BioTechnology: An Indian Journal >Study on the anti-quorum sensing activity of a marine bacterium Staphylococcus saprophyticus 108
【24h】

Study on the anti-quorum sensing activity of a marine bacterium Staphylococcus saprophyticus 108

机译：海洋细菌腐生葡萄球菌108的抗群体感应活性研究

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Quorum sensing inhibitors (QSI) may provide new research direction for the defense of bacterial diseases and biofilm related marine biofouling. QSI screening and development from natural source have received great interest in recent years. In this study, 17 marine bacterial strains, which were found exhibiting anti-quorum sensing activity in previous study, were secondary screened using pigment inhibition assay. One active bacterium 108 was identified as Staphylococcus saprophyticus based on the 16S rDNA sequence analysis and morphological observation. The culture condition of bacterium 108 was optimized. The results showed that moderate pH (6.0-7.0) and temperature (25°C) favored the bacterial growth and bioactive metabolites production. Yeast extracts content was the most importantmediumcomponent affecting the bacterial growth and bioactive metabolites production. By culturing under the optimized condition, the anti-quorum sensing activity of S. saprophyticus 108 and the yield of crude extracts was increased about 50% and 1.5 times, respectively, compared to the bacterial cultures before optimization. The active metabolites in S. saprophyticus 108 cultures were further extracted, isolated and identified. Cyclo (Pro-Leu), was identified from the active subfraction of the bacterium by GC-MS analysis. It was found for first time that Cyclo (Pro-Leu) exhibitedmoderate anti-quorumsensing activity.
机译：群体感应抑制剂（QSI）可能为防御细菌疾病和生物膜相关的海洋生物污染提供新的研究方向。近年来，从自然资源进行QSI筛选和开发引起了极大的兴趣。在这项研究中，使用色素抑制测定法对17株海洋细菌菌株进行了二次筛选，这些菌株在先前的研究中被发现具有抗群体感应活性。根据16S rDNA序列分析和形态学观察，鉴定出一种活性细菌108为腐生葡萄球菌。优化了细菌108的培养条件。结果表明，适度的pH（6.0-7.0）和温度（25°C）有利于细菌生长和生物活性代谢产物的产生。酵母提取物含量是影响细菌生长和生物活性代谢产物产生的最重要的中间成分。通过在优化条件下培养，与优化前的细菌培养相比，腐生链球菌108的抗群体感应活性和粗提物的收率分别提高了约50％和1.5倍。进一步提取，分离和鉴定了腐生链球菌108种培养物中的活性代谢物。通过GC-MS分析从细菌的活性亚组分中鉴定出Cyclo（Pro-Leu）。首次发现Cyclo（Pro-Leu）表现出中等的抗群体活性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号