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首页> 外文期刊>Biomedical Dermatology >Antiapoptotic effects of scutellarin on ultraviolet A-irradiated HaCaT human keratinocytes
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Antiapoptotic effects of scutellarin on ultraviolet A-irradiated HaCaT human keratinocytes

机译：黄cut苷对紫外线A照射的HaCaT人角质形成细胞的抗凋亡作用

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This study examines the cellular protective effects of scutellarin on HaCaT cells in which oxidative stress was induced by ultraviolet A (UVA). Cell viability and lipid peroxidation were measured using WST-1 and MDA assays, respectively. The changes in cell number in the sub-G1 phase were assessed using cell cycle analysis, and tailed DNA levels were measured using the comet assay to examine the degree of DNA damage. The difference in the mitochondrial membrane potential was examined using JC-1 staining, and the regulation of BAX mRNA expression levels was examined using quantitative real-time polymerase chain reaction. Although UVA-irradiated cells resulted in a 3.63-fold increase in lipid peroxide, scutellarin-treated cells showed concentration-dependent decreases of 2.06, 1.39, and 0.97 times at scutellarin concentrations of 1, 5, and 10 μM, respectively. The cell number in the sub-G1 phase increased by 227% when irradiated by UVA but decreased to 187, 139, and 120% when cells were treated with scutellarin at concentrations of 1, 5, and 10 μM, respectively. Tailed DNA also showed a protective effect. The mitochondrial membrane potential difference decreased up to 36% when irradiated by UVA but recovered up to 58, 82, and 92% when cells were treated with scutellarin at concentrations of 1, 5, and 10 μM, respectively. BAX gene expression levels increased 9.7-fold by UVA but was downregulated to 7.4-, 4.71-, and 2.49-fold when cells were treated with scutellarin at concentrations of 1, 5, and 10 μM, respectively. This study revealed the cellular protective effects of scutellarin on HaCaT cells. Further studies are warranted to determine the use of scutellarin as a cosmeceutical ingredient.
机译：这项研究研究了黄cut苷对HaCaT细胞的细胞保护作用，在HaCaT细胞中，紫外线A（UVA）诱导了氧化应激。分别使用WST-1和MDA测定法测量细胞活力和脂质过氧化作用。使用细胞周期分析评估sub-G1期中细胞数量的变化，并使用彗星测定法测量尾部DNA水平，以检查DNA损伤的程度。使用JC-1染色检查线粒体膜电位的差异，并使用定量实时聚合酶链反应检查BAX mRNA表达水平的调节。尽管经UVA照射的细胞导致脂质过氧化物增加了3.63倍，但经黄cut素处理的细胞在1,5、5和10μM的黄cut素浓度下，其浓度依赖性降低了2.06、1.39和0.97倍。用UVA照射时，sub-G1期的细胞数增加了227％，但是当分别以浓度分别为1,5和10μM的黄cut处理后，细胞数量减少到187、139和120％。尾状DNA也显示出保护作用。线粒体膜电位差在用UVA辐照时最多可降低36％，但在分别用浓度分别为1,5和10μM的黄cut素处理的细胞中最多可恢复58、82和92％。 BAX基因表达水平通过UVA增加9.7倍，但当分别用浓度分别为1,5和10μM的盾黄素处理细胞时，BAX基因表达水平被下调至7.4、4.71和2.49倍。这项研究揭示了黄cut苷对HaCaT细胞的细胞保护作用。有必要进行进一步的研究以确定黄素作为药妆成分的用途。

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