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首页> 外文期刊>BMC Biotechnology >Active fungal GH115 α-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans
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Active fungal GH115 α-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans

机译：拟南芥中产生的活性真菌GH115α-葡萄糖醛酸苷酶仅影响天然葡萄糖醛酸木聚糖上UX1反应性葡萄糖醛酸的装饰

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Background Expressing microbial polysaccharide-modifying enzymes in plants is an attractive approach to custom tailor plant lignocellulose and to study the importance of wall structures to plant development. Expression of α-glucuronidases in plants to modify the structures of glucuronoxylans has not been yet attempted. Glycoside hydrolase (GH) family 115 α-glucuronidases cleave the internal α-D-(4- O -methyl)glucopyranosyluronic acid ((Me)GlcA) from xylans or xylooligosaccharides. In this work, a GH115 α-glucuronidase from Schizophyllum commune, Sc AGU115, was expressed in Arabidopsis thaliana and targeted to apoplast. The transgene effects on native xylans’ structures, plant development, and lignocellulose saccharification were evaluated and compared to those of knocked out glucuronyltransferases At GUX1 and At GUX2.
机译：背景技术在植物中表达微生物多糖修饰酶是一种定制植物木质纤维素并研究壁结构对植物发育的重要性的有吸引力的方法。尚未尝试在植物中表达α-葡糖醛酸糖苷酶以修饰葡糖醛酸木聚糖的结构。糖苷水解酶（GH）家族115个α-葡萄糖醛酸糖苷酶从木聚糖或木寡糖中裂解内部的α-D-（4-O-甲基）吡喃葡萄糖基糖醛酸（（Me）GlcA）。在这项工作中，在拟南芥中表达了来自裂殖酵母属的GH115α-葡萄糖醛酸苷酶，Sc AGU115，并靶向了质外体。评估了转基因对天然木聚糖结构，植物发育和木质纤维素糖化的影响，并与在GUX1和At GUX2处剔除的葡萄糖醛酸转移酶进行了比较。

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