Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast

Milda Norkiene; Jomante Stonyte; Danguole Ziogiene; Egle Mazeike; Kestutis Sasnauskas; Alma Gedvilaite

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Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast

机译：从酵母中的新型人类多瘤病毒生产重组VP1衍生的病毒样颗粒

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Background Eleven new human polyomaviruses (HPyVs) have been identified in the last decade. Serological studies show that these novel HPyVs sub-clinically infect humans at an early age. The routes of infection, entry pathways, and cell tropism of new HPyVs remain unknown. VP1 proteins of polyomaviruses can assembly into virus-like particles (VLPs). As cell culturing systems for HPyV are currently not available, VP1-derived VLPs may be useful tools in basic research and biotechnological applications.

机译：背景技术在过去的十年中，已经鉴定出11种新的人类多瘤病毒（HPyV）。血清学研究表明，这些新颖的HPyVs在早期就亚临床感染人类。新型HPyV的感染途径，进入途径和细胞嗜性仍然未知。多瘤病毒的VP1蛋白可以组装成病毒样颗粒（VLP）。由于目前尚无用于HPyV的细胞培养系统，因此VP1衍生的VLP在基础研究和生物技术应用中可能是有用的工具。

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《BMC Biotechnology》 |2015年第1期|共页
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Milda Norkiene; Jomante Stonyte; Danguole Ziogiene; Egle Mazeike; Kestutis Sasnauskas; Alma Gedvilaite;
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中图分类生物工程学（生物技术）;
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1. Purification of recombinant trichodysplasia spinulosa-associated polyomavirus VP1-derived virus-like particles using chromatographic techniques [J] . Zaveckas Mindaugas, Goda Karolis, Ziogiene Danguole, Journal of chromatography, B. Analytical technologies in the biomedical and life sciences . 2018,第期

机译：使用色谱技术纯化重组序列胰蛋白酶分子瘤相关的多瘤VP1衍生的病毒样颗粒
2. Disassembly and reassembly of yeast-derived recombinant human papillomavirus virus-like particles (HPV VLPs). [J] . Mach H, Volkin DB, Troutman RD, Journal of pharmaceutical sciences. . 2006,第10期

机译：分解和重组酵母来源的重组人乳头瘤病毒样病毒（HPV VLP）。
3. Production and biomedical applications of virus-like particles derived from polyomaviruses [J] . Teunissen E.A., De Raad M., Mastrobattista E. Journal of Controlled Release: Official Journal of the Controlled Release Society . 2013,第1期

机译：多瘤病毒衍生的病毒样颗粒的生产和生物医学应用
4. Characterization of Inter-capsomeric Disulfide Bonding in Human Papillomavirus Recombinant Virus-like Particles Using LC/MS [C] . Colleen Price, Marc Wenger, Lorenzo Chen, ASMS Conference on Mass Spectrometry and Allied Topics . 2006

机译：使用LC / MS的人乳头瘤病毒重组病毒样颗粒中囊囊间二硫键的表征
5. Identification and characterization of a heparin-binding region on the L1 major capsid protein of recombinant human papillomavirus virus-like particles and its role in interactions of VLPs with cells. [D] . Joyce, Joseph Gerard. 1998

机译：重组人乳头瘤病毒病毒样颗粒的L1主要衣壳蛋白上肝素结合区的鉴定和表征及其在VLP与细胞相互作用中的作用。
6. Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast [O] . Milda Norkiene, Jomante Stonyte, Danguole Ziogiene, 2015

机译：从酵母中的新型人类多瘤病毒生产重组VP1衍生的病毒样颗粒
7. Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast [O] . Milda Norkiene, Jomante Stonyte, Danguole Ziogiene, 2015

机译：从酵母中的新型人类多瘤病毒生产重组VP1衍生的病毒样颗粒

Production of recombinant VP1-derived virus-like particles from novel human polyomaviruses in yeast

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