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首页> 外文期刊>BMC Biotechnology >Feasibility of Cowpea chlorotic mottle virus-like particles as scaffold for epitope presentations
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Feasibility of Cowpea chlorotic mottle virus-like particles as scaffold for epitope presentations

机译：Cow豆绿叶斑驳病毒样颗粒作为表位展示支架的可行性

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Background & Methods Within the last decade Virus-Like Particles (VLPs) have increasingly received attention from scientists for their use as a carrier of (peptide) molecules or as scaffold to present epitopes for use in subunit vaccines. To test the feasibility of Cowpea chlorotic mottle virus (CCMV) particles as a scaffold for epitope presentation and identify sites for epitope fusion or insertion that would not interfere with virus-like-particle formation, chimeric CCMV coat protein (CP) gene constructs were engineered, followed by expression in E. coli and assessment of VLP formation. Various constructs were made encoding a 6x-His-tag, or selected epitopes from Influenza A virus [IAV] (M2e, HA) or Foot and Mouth Disease Virus [FMDV] (VP1 and 2C). The epitopes were either inserted 1) in predicted exposed loop structures of the CCMV CP protein, 2) fused to the amino- (N) or carboxyl-terminal (C) ends, or 3) to a N-terminal 24 amino acid (aa) deletion mutant (N?24-CP) of the CP protein.
机译：背景与方法在过去十年中，像病毒样颗粒（VLP）越来越多地受到科学家的关注，因为它们可用作（肽）分子的载体或用作展示亚基疫苗中使用的表位的支架。为了测试Cow豆绿斑驳斑驳病毒（CCMV）颗粒作为表位呈递支架的可行性，并确定不会干扰病毒样颗粒形成的表位融合或插入位点，设计了嵌合CCMV外壳蛋白（CP）基因构建体，然后在大肠杆菌中表达并评估VLP形成。制备了多种编码6x-His标签的构建体，或选自甲型流感病毒[IAV]（M2e，HA）或口蹄疫病毒[FMDV]（VP1和2C）的选定表位。将表位插入1）在CCMV CP蛋白的预计暴露环结构中，2）与氨基（N）或羧基末端（C）末端融合或3）与N末端的24个氨基酸（aa）融合）CP蛋白的缺失突变体（N？24-CP）。

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