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首页> 外文期刊>BMC Biotechnology >Economic co-production of poly(malic acid) and pullulan from Jerusalem artichoke tuber by Aureobasidium pullulans HA-4D
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Economic co-production of poly(malic acid) and pullulan from Jerusalem artichoke tuber by Aureobasidium pullulans HA-4D

机译：Aureobasidium Pullulans HA-4D从菊芋块茎经济联产聚苹果酸和支链淀粉

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Backgroundpoly(L-malic acid) (PMA) is a water-soluble polyester with many attractive properties in medicine and food industries, but the high cost of PMA fermentation has restricted its further application for large-scale production. To overcome this problem, PMA production from Jerusalem artichoke tubers was successfully performed. Additionally, a valuable exopolysaccharide, pullulan, was co-produced with PMA by Aureobasidum pullulans HA-4D. ResultsThe Jerusalem artichoke medium for PMA and pullulan co-production contained only 100?g/L hydrolysate sugar, 30?g/L CaCO3 and 1?g/L NaNO3. Compared with the glucose medium, the Jerusalem artichoke medium resulted in a higher PMA concentration (114.4?g/L) and a lower pullulan concentration (14.3?g/L) in a 5?L bioreactor. Meanwhile, the activity of pyruvate carboxylase and malate dehydrogenas was significantly increased, while the activity of α-phosphoglucose mutase, UDP-glucose pyrophosphorylase and glucosyltransferase was not affected. To assay the economic-feasibility, large-scale production in a 1?t fermentor was performed, yielding 117.5?g/L PMA and 15.2?g/L pullulan. ConclusionsIn this study, an economical co-production system for PMA and pullulan from Jerusalem artichoke was developed. The medium for PMA and pullulan co-production was significantly simplified when Jerusalem artichoke tubers were used. With the simplified medium, PMA production was obviously stimulated, which would be associated with the improved activity of pyruvate carboxylase and malate dehydrogenas.
机译：背景聚（L-苹果酸）（PMA）是在医学和食品工业中具有许多吸引人的特性的水溶性聚酯，但是PMA发酵的高成本限制了其在大规模生产中的进一步应用。为了克服这个问题，成功进行了菊芋块茎的PMA生产。另外，一种有价值的胞外多糖，支链淀粉，是由Aureobasidum pullulans HA-4D与PMA共同生产的。结果用于PMA和支链淀粉联产的菊芋培养基中仅含100？g / L水解糖，30？g / L CaCO 3 和1？g / L NaNO 3 。与葡萄糖培养基相比，菊芋培养基在5 µL生物反应器中导致较高的PMA浓度（114.4 µg / L）和较低的支链淀粉浓度（14.3 µg / L）。同时，丙酮酸羧化酶和苹果酸脱氢酶的活性显着增加，而α-磷酸葡萄糖变位酶，UDP-葡萄糖焦磷酸化酶和葡萄糖基转移酶的活性未受影响。为了测定经济可行性，在1升发酵罐中进行了大规模生产，产量为117.5微克/升PMA和15.2微克/升支链淀粉。结论在这项研究中，开发了经济的联合生产菊芋中的PMA和支链淀粉的产品。使用菊芋块茎显着简化了PMA和支链淀粉联产的培养基。通过简化的培养基，明显刺激了PMA的产生，这与丙酮酸羧化酶和苹果酸脱氢酶活性的提高有关。

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