• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Biotechnology >Identification of two transcription factors activating the expression of OsXIP in rice defence response
【24h】

Identification of two transcription factors activating the expression of OsXIP in rice defence response

机译：鉴定激活水稻防御反应中OsXIP表达的两种转录因子

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BackgroundXylanase inhibitors have been confirmed to be involved in plant defence. OsXIP is a XIP-type rice xylanase inhibitor, yet its transcriptional regulation remains unknown. ResultsHerbivore infestation, wounding and methyl jasmonate (MeJA) treatment enhanced mRNA levels and protein levels of OsXIP. By analyzing different 5’ deletion mutants of OsXIP promoter exposed to rice brown planthopper Nilaparvata lugens stress, a 562?bp region (–1451 – ?889) was finally identified as the key sequence for the herbivores stress response. Using yeast one-hybrid screening, coupled with chromatin immunoprecipitation analysis, a basic helix-loop-helix protein (OsbHLH59) and an APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factor OsERF71 directly binding to the 562?bp key sequence to activate the expression of OsXIP were identified, which is further supported by transient expression assay. Moreover, transcriptional analysis revealed that mechanical wounding and treatment with MeJA resulted in an obvious increase in transcript levels of OsbHLH59 and OsERF71 in root and shoot tissues. ConclusionsOur data shows that two proteins as direct transcriptional activators of OsXIP responding to stress were identified. These results reveal a coordinated regulatory mechanism of OsXIP, which may probably be involved in defence responses via a JA-mediated signaling pathway.
机译：背景木聚糖酶抑制剂已被证实参与植物防御。 OsXIP是一种XIP型水稻木聚糖酶抑制剂，但其转录调控仍然未知。结果草茎虫侵染，创伤和茉莉酸甲酯（MeJA）处理增强了OsXIP的mRNA水平和蛋白质水平。通过分析暴露于水稻褐飞虱Nilaparvata lugens胁迫下的OsXIP启动子的5'缺失突变体，最终确定了562bp的区域（–1451 – 889）是草食动物胁迫反应的关键序列。使用酵母一杂交筛选，结合染色质免疫沉淀分析，基本的螺旋-环-螺旋蛋白（OsbHLH59）和APETALA2 /乙烯响应因子（AP2 / ERF）转录因子OsERF71直接结合到562？bp的关键序列上进行激活鉴定了OsXIP的表达，这由瞬时表达测定进一步支持。此外，转录分析显示，机械损伤和MeJA处理导致根和芽组织中OsbHLH59和OsERF71的转录水平明显增加。结论我们的数据表明，鉴定出了两种蛋白作为OsXIP的直接转录激活因子，可以响应压力。这些结果揭示了OsXIP的协调调节机制，可能通过JA介导的信号通路参与防御反应。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号