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首页> 外文期刊>BMC Biotechnology >Factors affecting polyhydroxybutyrate accumulation in mesophyll cells of sugarcane and switchgrass
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Factors affecting polyhydroxybutyrate accumulation in mesophyll cells of sugarcane and switchgrass

机译：影响甘蔗和柳枝me叶肉细胞中多羟基丁酸酯积累的因素

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Background Polyhydroxyalkanoates are linear biodegradable polyesters produced by bacteria as a carbon store and used to produce a range of bioplastics. Widespread polyhydroxyalkanoate production in C4 crops would decrease petroleum dependency by producing a renewable supply of biodegradable plastics along with residual biomass that could be converted into biofuels or energy. Increasing yields to commercial levels in biomass crops however remains a challenge. Previously, lower accumulation levels of the short side chain polyhydroxyalkanoate, polyhydroxybutyrate (PHB), were observed in the chloroplasts of mesophyll (M) cells compared to bundle sheath (BS) cells in transgenic maize ( Zea mays ), sugarcane ( Saccharum sp.), and switchgrass ( Panicum virgatum L.) leading to a significant decrease in the theoretical yield potential. Here we explore various factors which might affect polymer accumulation in mesophyll cells, including targeting of the PHB pathway enzymes to the mesophyll plastid and their access to substrate. Results The small subunit of Rubisco from pea effectively targeted the PHB biosynthesis enzymes to both M and BS chloroplasts of sugarcane and switchgrass. PHB enzyme activity was retained following targeting to M plastids and was equivalent to that found in the BS plastids. Leaf total fatty acid content was not affected by PHB production. However, when fatty acid synthesis was chemically inhibited, polymer accumulated in M cells. Conclusions In this study, we provide evidence that access to substrate and neither poor targeting nor insufficient activity of the PHB biosynthetic enzymes may be the limiting factor for polymer production in mesophyll chloroplasts of C4 plants.
机译：背景技术聚羟基链烷酸酯是由细菌作为碳存储物生产的线性可生物降解的聚酯，并用于生产一系列生物塑料。在C 4 作物中广泛生产聚羟基链烷酸酯，将产生可再生的可生物降解塑料以及残余生物质，这些残余生物可转化为生物燃料或能源，从而降低了对石油的依赖。然而，将生物质作物的单产提高到商业水平仍然是一个挑战。以前，在转基因玉米（Zea mays），甘蔗（Saccharum sp。）中，与叶鞘（BS）细胞相比，叶肉（M）细胞的叶绿体中观察到了短侧链多羟基链烷酸酯，多羟基丁酸酯（PHB）较低的积累水平。和柳枝（（Panicum virgatum L.）导致理论产量潜力显着下降。在这里，我们探讨了可能影响叶肉细胞中聚合物积累的各种因素，包括将PHB途径酶靶向至叶肉质体及其进入底物的途径。结果来自豌豆的Rubisco小亚基有效地将PHB生物合成酶靶向于甘蔗和柳枝M的M和BS叶绿体。靶向M质体后，PHB酶的活性得以保留，与BS质体中的活性相当。叶片总脂肪酸含量不受PHB产生的影响。但是，当脂肪酸的合成受到化学抑制时，聚合物会积聚在M细胞中。结论在这项研究中，我们提供的证据表明，接近底物，PHB生物合成酶的靶向性不佳或活性不足可能是C 4 植物叶肉叶绿体中聚合物生产的限制因素。

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