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首页> 外文期刊>BMC Biotechnology >Mesenchymal stem cell spheroids exhibit enhanced in-vitro and in-vivo osteoregenerative potential
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Mesenchymal stem cell spheroids exhibit enhanced in-vitro and in-vivo osteoregenerative potential

机译：间充质干细胞球体具有增强的体外和体内骨再生潜能

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Background Mesenchymal stem cells (MSCs) are a favored cell source for regenerative medicine because of their multilinage potential. However, the conventional monolayer technique used to culture MSCs, inadequately overcomes their low differentiation capacity. Culture of MSCs in multicellular spheroids, more accurately mimics the in-vivo microenvironment; thus, resolving this problem. In this study, we assessed whether the osteoregenerative potential of MSC spheroids is greater than that of monolayer MSCs. Results MSC spheroids were generated from rat MSCs (rMSCs) using low-binding plates. Real-time reverse transcription-polymerase chain reaction and immunocytochemical analysis indicated that osteogenic properties were accelerated in MSC spheroids compared with monolayer rMSCs when treated with an osteoblast-inducer reagent for 7?days. Moreover, increased calcium deposition was visualized in MSC spheroids using Alizarin red staining. In a rat calvarial defect model, micro-computed tomography and histological assays showed that MSC spheroid-engrafted defects experienced enhanced bone regeneration. Conclusions Our in-vitro and in-vivo results reveal that MSCs in the spheroid culture exhibit enhanced osteoregenerative efficiency compared with monolayer MSCs.
机译：背景间充质干细胞（MSCs）由于具有多种用途，因此是再生医学的首选细胞来源。然而，用于培养MSC的常规单层技术不足以克服它们的低分化能力。在多细胞球体中培养MSC，可以更准确地模拟体内微环境;因此，解决了这个问题。在这项研究中，我们评估了MSC球体的骨再生潜力是否大于单层MSC。结果MSC球体是使用低结合板从大鼠MSC（rMSC）生成的。实时逆转录-聚合酶链反应和免疫细胞化学分析表明，当用成骨细胞诱导剂处理7天时，与单层rMSC相比，MSC球体的成骨特性得到了加速。此外，使用茜素红染色可观察到MSC球体中钙沉积的增加。在大鼠颅骨缺损模型中，显微计算机断层扫描和组织学分析表明，MSC球状植入的缺损经历了增强的骨再生。结论我们的体外和体内结果表明，与单层MSC相比，球状培养物中的MSC具有更高的骨再生效率。

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