• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Biotechnology >Saccharification of rice straw by cellulase from a local Trichoderma harzianum SNRS3 for biobutanol production
【24h】

Saccharification of rice straw by cellulase from a local Trichoderma harzianum SNRS3 for biobutanol production

机译：用当地哈茨木霉SNRS3的纤维素酶将稻草糖化以生产生物丁醇

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Rice straw has shown to be a promising agricultural by-product in the bioconversion of biomass to value-added products. Hydrolysis of cellulose, a main constituent of lignocellulosic biomass, is a requirement for fermentable sugar production and its subsequent bioconversion to biofuels such as biobutanol. The high cost of commercial enzymes is a major impediment to the industrial application of cellulases. Therefore, the use of local microbial enzymes has been suggested. Trichoderma harzianum strains are potential CMCase and β-glucosidase producers. However, few researches have been reported on cellulase production by T. harzianum and the subsequent use of the crude cellulase for cellulose enzymatic hydrolysis. For cellulose hydrolysis to be efficiently performed, the presence of the whole set of cellulase components including exoglucanase, endoglucanase, and β-glucosidase at a considerable concentration is required. Biomass recalcitrance is also a bottleneck in the bioconversion of agricultural residues to value-added products. An effective pretreatment could be of central significance in the bioconversion of biomass to biofuels. Results Rice straw pretreated using various concentrations of NaOH was subjected to enzymatic hydrolysis. The saccharification of rice straw pretreated with 2% (w/v) NaOH using crude cellulase from local T. harzianum SNRS3 resulted in the production of 29.87 g/L reducing sugar and a yield of 0.6 g/g substrate. The use of rice straw hydrolysate as carbon source for biobutanol fermentation by Clostridium acetobutylicum ATCC 824 resulted in an ABE yield, ABE productivity, and biobutanol yield of 0.27 g/g glucose, 0.04 g/L/h and 0.16 g/g glucose, respectively. As a potential β-glucosidase producer, T. harzianum SNRS3 used in this study was able to produce β-glucosidase at the activity of 173.71 U/g substrate. However, for cellulose hydrolysis to be efficient, Filter Paper Activity at a considerable concentration is also required to initiate the hydrolytic reaction. According to the results of our study, FPase is a major component of cellulose hydrolytic enzyme complex system and the reducing sugar rate-limiting enzyme. Conclusion Our study revealed that rice straw hydrolysate served as a potential substrate for biobutanol production and FPase is a rate-limiting enzyme in saccharification.
机译：背景技术稻草已经显示出是将生物质生物转化为增值产品的有前途的农业副产品。纤维素的水解是木质纤维素生物质的主要成分，是可发酵糖生产及其随后生物转化为生物燃料（如生物丁醇）的要求。商业酶的高成本是纤维素酶工业应用的主要障碍。因此，已经建议使用局部微生物酶。哈茨木霉菌株是潜在的CMCase和β-葡萄糖苷酶生产者。然而，关于哈茨木霉生产纤维素酶以及随后将粗纤维素酶用于纤维素酶水解的报道很少。为了有效地进行纤维素水解，需要以相当大的浓度存在包括外切葡聚糖酶，内切葡聚糖酶和β-葡糖苷酶的整套纤维素酶组分。生物质顽固性也是农业残留物生物转化为增值产品的瓶颈。有效的预处理对于将生物质转化为生物燃料具有重要意义。结果使用各种浓度的NaOH预处理的稻草进行了酶水解。使用来自本地哈茨木霉SNRS3的粗纤维素酶对稻草进行2％（w / v）NaOH的糖化处理，可产生29.87 g / L的还原糖，产量为0.6 g / g的底物。使用稻草水解物作为丙酮丁醇梭菌ATCC 824发酵生物丁醇的碳源时，其ABE产量，ABE生产率和生物丁醇产量分别为0.27 g / g葡萄糖，0.04 g / L / h和0.16 g / g葡萄糖。 。作为潜在的β-葡萄糖苷酶生产者，本研究中使用的哈茨木霉SNRS3能够以173.71 U / g底物的活性产生β-葡萄糖苷酶。然而，为了使纤维素水解有效，还需要相当浓度的滤纸活性以引发水解反应。根据我们的研究结果，FPase是纤维素水解酶复合物系统的主要成分，也是还原糖速率限制酶。结论我们的研究表明稻草水解物可作为生物丁醇生产的潜在底物，而FPase是糖化过程中的限速酶。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号