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The effect of Melaleuca cajuputi methanolic leaves extract on body growth, puberty and sperm quality of juvenile male rats

机译:白千层甲醇叶提取物对幼年雄性大鼠身体生长,青春期和精子质量的影响

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The aims of this study was to determine the effects of Melaleuca cajuputi methanolic leaves extract on body growth, the timing of puberty and sperm quality in juvenile rats. Twenty four Sprague Dawleymale rats, four weeks old, were assigned to four different groups (n=6). The animals received standard maintenance diets supplemented with different levels of M. cajuputi methanolic leaves extract. First group served as a control and only received maintenance diets. Meanwhile another three groups supplementedwith 50, 100 and 200mg.kg-1 ofM. cajuputimethanolic leaves extract, respectively, for sixweeks. Bodyweight and the degree of preputial separation were monitored on weekly basis.At the end of the experiment, all animals were sacrificed and their epidydimis were subjected to sperm analysis. Over six weeks of experiment, the bodyweight increased significantly (p 0.05) was observed between treatment. The number of male with fully preputial separation was significantly (p<0.05) higher in males supplemented with 200 mg.kg-1 of M. cajuputi. The sperm quality was increased with M. cajuputi supplementation. These results suggest that the M. cajuputi methanolic leaves extract improved sperm quality and reduced the age to achieve puberty in male.
机译:这项研究的目的是确定千层甲醇叶提取物对幼年大鼠的身体生长,青春期的时间和精子质量的影响。将四周大的24只Sprague Dawleymale大鼠分成四个不同的组(n = 6)。这些动物接受了标准的维持饮食,并补充了不同含量的Cajuputi甲醇叶提取物。第一组作为对照,仅接受维持饮食。同时,另外三组分别补充了50、100和200mg.kg-1的M。 cajuputimethanolic叶子提取物分别为期六周。每周监测体重和包皮分离程度。在实验结束时,处死所有动物并对其附睾进行精子分析。经过六周的实验,治疗之间体重显着增加(p 0.05)。补充了200 mg.kg-1的卡氏假丝酵母的男性中,具有完全外皮分离的男性数量显着增加(p <0.05)。补充Ca.putu M.可提高精子质量。这些结果表明,南美番荔枝甲醇叶提取物改善了精子质量,并降低了男性达到青春期的年龄。

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