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首页> 外文期刊>BioTechnology: An Indian Journal >Hepcidin-25 a well iron biomarker with prognostic implications in chronic kidney diseases
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Hepcidin-25 a well iron biomarker with prognostic implications in chronic kidney diseases

机译：Hepcidin-25是一种良好的铁生物标志物，对慢性肾脏疾病具有预后意义

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AIM: Hepcidin is a 25-aminoacid cysteine-rich iron regulating peptide. Hepcidin quantification in human serumprovides newtopics for the pathogenesis of disorders of iron homeostasis and its treatment. This study describes ELISA immunoassay for hepcidin quantification in human serumin chronic kidney disease. DATA:We use a sandwich ELISAmethod to quantificate serum hepcidin levels in healthy control group (n=60) and patients with chronic kidney disease (n=34). Including criteria for control group was no evidence of ironmetabolismdisorders.RESULTS:The sandwich ELISA was highly specific for hepcidin-25. We found that serum hepcidin levels correlate significantly between two groups 13.1 ± 8.7 μg/L to 90.7 ± 74.2 μg/L. Ferritin levels and hemoglobin concentration in reticulocytes correlated significantly to serum hepcidin levels (0.3 < r <0.5, p < 0.010). Transferrine levels showed negative and no significant correlation to hepcidin in serum(r=-0.111). CONCLUSIONS: The use of 2 monoclonal antibodies in a sandwich ELISA format provides a reliable, reproducible and not very expensive method for measuring serum concentrations of the bioactive form of hepcidin in laboratory practice.
机译：目的：铁调素是一种25个氨基酸的富含半胱氨酸的铁调节肽。人血清中的铁调素定量为铁体内稳态疾病的发病机理及其治疗提供了新的话题。这项研究描述了用于慢性肾脏病患者血清中铁调素定量的ELISA免疫测定。数据：我们使用三明治ELISA方法对健康对照组（n = 60）和慢性肾脏病患者（n = 34）的血清hepcidin水平进行定量。结果：夹心ELISA对hepcidin-25具有高度特异性。我们发现两组血清血清铁调素水平显着相关13.1±8.7μg/ L至90.7±74.2μg/ L。网织细胞中铁蛋白水平和血红蛋白浓度与血清铁调素水平显着相关（0.3

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