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首页> 外文期刊>BioTechnology: An Indian Journal >Evaluation and characterization of polyhydroxybutyrate produced by Azotobacter chroococcum
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Evaluation and characterization of polyhydroxybutyrate produced by Azotobacter chroococcum

机译：绿脓杆菌产生的多羟基丁酸酯的评价和表征

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Production and characterization of biodegradable plastic namely polyhydroxybutyrate (PHB) in Azotobacter chroococcumhave been carried out using different carbon sources such as: ethanol, sugar cane molasses in comparison with glucose leading to develop a low cost process of PHB production. The accumulation of PHB granule in cells of A. chroococcum was significantly dependent on the content of carbon sources in themedium culture. In the case of using ethanol, the PHB producedwas high significant percentage (42 w/w%) in comparison with molasses and glucose (31 and 16 w/w %, respectively). Light, transmission and scanning electron microscopes (TEM and SEM) images of the staining cysts illustrated that different morphologies were obtained relative to that in the case of the vegetative one.Moreover, the physicochemical properties of the produced PHB were studied and evaluated using different analytical tools such as: Fourier transforminfrared spectroscopy (FTIR), nuclearmagnetic resonance spectroscopy (1H-NMR),X-ray diffraction pattern (XRD), high performance liquid chromatography (HPLC) and differential scanning calorimetry (DSC).
机译：已使用不同的碳源（如乙醇，甘蔗糖蜜和葡萄糖）与不同的碳源在蓝细菌中生产和表征了可生物降解塑料，即聚羟基丁酸酯（PHB），从而开发了低成本的PHB生产方法。嗜铬曲霉细胞中PHB颗粒的积累显着取决于主题培养中碳源的含量。在使用乙醇的情况下，与糖蜜和葡萄糖（分别为31和16 w / w％）相比，所产生的PHB具有很高的显着百分比（42 w / w％）。染色囊肿的光镜，透射电镜和扫描电镜（TEM和SEM）图像表明，与营养性囊肿相比，形态不同。此外，还研究了PHB的理化性质并使用不同的分析方法对其进行了评估。工具包括：傅立叶变换红外光谱（FTIR），核磁共振光谱（1H-NMR），X射线衍射图（XRD），高效液相色谱（HPLC）和差示扫描量热法（DSC）。

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