首页> 外文期刊>BioTechnology: An Indian Journal >In vitro organogenesis and plantlet regeneration of pigeonpea [Cajanus cajan (L.) Millsp.]
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In vitro organogenesis and plantlet regeneration of pigeonpea [Cajanus cajan (L.) Millsp.]

机译:木豆的体外器官发生和植株再生[Cajanus cajan(L.)Millsp。]

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Adventitious shoot regenerationwas achieved fromepicotyl and internode explants of in vitro grown seedlings of Cajanus cajan.Maximumnumber of multiple shoot formation was obtained by culturing the above explants on MS medium containing 1.0 mg/l BA and 0.5 mg/l NAA. The microcuttingswere developed to complete plantlets after transferring them to the rooting media supplementation with auxins namely, IBA, NAA or IAA. Nevertheless, 0.1-0.2 mg/l IBA and NAA was found to be most suitable for root induction. The microplantswere successfully established in the soil under natural environment with about 75% survival.
机译:体外培养的木豆幼苗的表皮和节间外植体实现了不定芽的再生。将上述外植体在含有1.0 mg / l BA和0.5 mg / l NAA的MS培养基上培养可获得最大的多芽形成量。在将小苗转移到补充了生长素(IBA,NAA或IAA)的生根培养基后,将其切成小苗。然而,发现0.1-0.2 mg / l IBA和NAA最适合根诱导。在自然环境下成功地在土壤中建立了微型植物,其存活率约为75%。

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