Functional evaluation of ToxA promoter in Trichoderma reeseiRut-C30

Ortiz E.Gast???3n; Albert???3 Edgardo; BlascoMart???-n

首页> 外文期刊>BioTechnology: An Indian Journal >Functional evaluation of ToxA promoter in Trichoderma reeseiRut-C30

【24h】

Functional evaluation of ToxA promoter in Trichoderma reeseiRut-C30

机译：ToxA启动子在里氏木霉C30中的功能评估

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

In order to expand the collection of promoters studied in Trichoderma reesei Rut-C30, a functional study of the ToxA promoter was carried out by analysis of GFP expression in T. reesei Rut-C30. For this purpose, the binary pCBCT expression vectorwas employed to transformT. reesei Rut- C30. The transformants obtained were evaluated bymeans of fluorescence microscopy, fluorometry, dot blot andWestern blot analysis. The lowlevels of cytoplasmic GFP protein in fungal hyphae suggest that ToxA promoter works as a weak constitutive promoter which can drive successfully the expression of heterologous proteins in T. reesei Rut-C30.

机译：为了扩大在里氏木霉Rut-C30中研究的启动子的收集，通过分析里氏木霉Rut-C30中的GFP表达来进行ToxA启动子的功能研究。为此目的，使用二进制pCBCT表达载体来转化T。里斯Rut- C30。通过荧光显微镜，荧光法，斑点印迹和Western印迹分析的手段评估获得的转化体。真菌菌丝中的胞质GFP蛋白水平较低，表明ToxA启动子是弱组成型启动子，可成功驱动里氏木霉Rut-C30中异源蛋白的表达。

著录项

来源
《BioTechnology: An Indian Journal》 |2016年第1期|共6页
作者
Ortiz E.Gast???3n; Albert???3 Edgardo; BlascoMart???-n;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类生物工程学（生物技术）;
关键词
TrichodermareeseiRut-C30Recombinant protein expressionToxA promoterWeak promoter;

机译：木霉木霉Rut-C30重组蛋白表达ToxA启动子弱启动子;

相似文献

外文文献
中文文献
专利

1. Evaluation and characterization of Trichoderma reesei cellulase and xylanase promoters [J] . Rahman Z., Shida Y., Furukawa T., Applied Microbiology and Biotechnology . 2009,第5期

机译：里氏木霉纤维素酶和木聚糖酶启动子的评估和表征
2. Cloning, functional expression and promoter analysis of xylanase III gene from Trichoderma reesei [J] . Ogasawara W, Shida Y, Furukawa T, Applied Microbiology and Biotechnology . 2006,第5期

机译：里氏木霉木聚糖酶III基因的克隆，功能表达及启动子分析
3. Cloning, functional expression and promoter analysis of xylanase III gene from Trichoderma reesei [J] . W. Ogasawara, Y. Shida, T. Furukawa, Applied Microbiology and Biotechnology . 2006,第5期

机译：里氏木霉木聚糖酶III基因的克隆，功能表达及启动子分析
4. Application of Trichoderma harzianum (T22) and Trichoderma atroviride (P1) as plant growth promoters, and their compatibility with copper oxychloride [C] . International Workshop on Trichoderma and Gliocladium; ; . 2004

机译：哈茨木霉（T22）和阿特木霉（P1）作为植物生长促进剂的应用及其与氯氧化铜的相容性
5. Functional analysis of rice bidirectional promoters. [D] . Dhadi, Surendar R. 2012

机译：水稻双向启动子的功能分析。
6. Evaluation of a functional epigenetic approach to identify promoter region methylation in phaeochromocytoma and neuroblastoma [O] . Caroline D E Margetts, Mark Morris, Dewi Astuti, -1

机译：评估功能性表观遗传方法以鉴定嗜铬细胞瘤和神经母细胞瘤中启动子区域的甲基化
7. Evaluation of the Potential of Trichoderma harzianum as a Plant Growth Promoter and Biocontrol Agent Against Fusarium Damping-off in Onion in Burkina Faso [O] . T.G. Dabire, S. Bonzi, I. Somda, 2016

机译：评估Trichoderma harzianum作为植物生长促进剂和Biocontrol在Burkina Faso的洋葱中抑制镰刀菌的潜力
8. Functionalized block copolymers as adhesion promoters [R] . Kent, M. S. , Saunders, R. 1995

机译：官能化嵌段共聚物作为粘合促进剂

Functional evaluation of ToxA promoter in Trichoderma reeseiRut-C30

摘要

著录项

相似文献

相关主题

期刊订阅