High-level expression of human arginase I in Pichia pastoris and its immobilization on chitosan to produce L-ornithine

Xue Zhang; Jin Liu; Xianhong Yu; Fei Wang; Li Yi; Zhezhe Li; Yunyun Liu; Lixin Ma

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High-level expression of human arginase I in Pichia pastoris and its immobilization on chitosan to produce L-ornithine

机译：人精氨酸酶I在巴斯德毕赤酵母中的高表达及其在壳聚糖上的固定化以产生L-鸟氨酸

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Background L-ornithine (L-Orn), is an intermediate metabolite in the urea cycle that plays a significant role in humans. L-Orn can be obtained from the catalysis of L-arginine (L-Arg) by arginase. The Pichia pastoris expression system offers the possibility of generating a large amount of recombinant protein. The immobilized enzyme technology can overcome the difficulties in recovery, recycling and long-term stability that result from the use of free enzyme.

机译：背景L-鸟氨酸（L-Orn）是尿素循环中的一种中间代谢产物，在人类中起着重要作用。 L-Orn可由精氨酸酶催化L-精氨酸（L-Arg）获得。毕赤酵母表达系统提供了产生大量重组蛋白的可能性。固定化酶技术可以克服由于使用游离酶而导致的回收，再循环和长期稳定性方面的困难。

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《BMC Biotechnology》 |2015年第1期|共页
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Xue Zhang; Jin Liu; Xianhong Yu; Fei Wang; Li Yi; Zhezhe Li; Yunyun Liu; Lixin Ma;
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中图分类生物工程学（生物技术）;
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2. High-level expression and characterization of a highly thermostable chitosanase from Aspergillus fumigatus in Pichia pastoris. [J] . Xiaomei Chen Chao Zhai Lixin Kang Chunhua Li Hong Yan Yuling Zhou Xiaolan Yu Lixin Ma Biotechnology Letters . 2012,第4期

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3. High-level expression and characterization of a highly thermostable chitosanase from Aspergillus fumigatus in Pichia pastoris [J] . Xiaomei Chen, Chao Zhai, Lixin Kang, Biotechnology Letters . 2012,第4期

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4. Secretory Expression of Insulin Precursor in Pichia Pastoris and Simple Procedure for Producing Recombinant Human Insulin [C] . The 12th Asian Pacific Confederation of Chemical Engineering Congress(第十二届亚太化工联盟大会暨化工展览会)论文集 . 2008

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5. The human T-cell leukemia virus type-II integrase: Studies in DNA recognition and recombinant protein expression (Pichia pastoris). [D] . Piefer, Andrew John. 2001

机译：II型人类T细胞白血病病毒整合酶：DNA识别和重组蛋白表达（巴斯德毕赤酵母）的研究。
6. High-level expression of human arginase I in Pichia pastoris and its immobilization on chitosan to produce L-ornithine [O] . Xue Zhang, Jin Liu, Xianhong Yu, 2015

机译：人精氨酸酶I在巴斯德毕赤酵母中的高表达及其在壳聚糖上的固定化以产生L-鸟氨酸
7. High-level expression of human arginase I in Pichia pastoris and its immobilization on chitosan to produce L-ornithine [O] . 2015

机译：人精氨酸酶I在巴斯德毕赤酵母中的高表达及其在壳聚糖上的固定化以产生L-鸟氨酸

High-level expression of human arginase I in Pichia pastoris and its immobilization on chitosan to produce L-ornithine

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