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首页> 外文期刊>BioTechnology: An Indian Journal >Enzymatic transformation of Panax notoginseng stem and leaves for preparation anti-tumor activity constituents
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Enzymatic transformation of Panax notoginseng stem and leaves for preparation anti-tumor activity constituents

机译：三七茎叶的酶促转化制备抗肿瘤活性成分

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Ginsenoside bioactive compounds, namely, 20(S)-protopanaxadiol-20-O- a-D-glucopyranoside (Compound-K,C-K) and 20(S)-protopanaxadiol-20- O-a-D-glucopyranoside (compound Mc, G-Mc) were converted from the saponins separated from the stem and leaves wastes of P. notoginseng (PNLS) by the industrial enzyme. The enzymatic transformed products CK and G-Mc were extracted byD-101macroreticular resins, separated and purified by column chromatographies on silica gel and thin layer gel, and their structures were confirmed by 1H and 13C NMR analysis. Under the optimumenzymatic transformation conditions of pH5.4, 55???°C, 20mgmL-1of PNLS and 10%(v/v) of ?-glucanase, 88.30mg g-1 C-K and 10.21 mg g-1GMc was produced after 48 h.
机译：将人参皂苷生物活性化合物即20（S）-原人参二醇20-O-aD-吡喃葡萄糖苷（化合物K，CK）和20（S）-原人参二醇-20-OaD-吡喃葡萄糖苷（化合物Mc，G-Mc）转化从工业茎中分离出的皂苷从茎中分离出来，并留下三七的废料。用D-101大网状树脂提取酶促转化产物CK和G-Mc，在硅胶和薄层凝胶上通过柱色谱法分离纯化，并通过1H和13C NMR分析确定其结构。在最佳酶促转化条件为pH5.4、55°C，20mgmL-1的PNLS和10％（v / v）的β-葡聚糖酶的作用下，经过48天的反应，产生了88.30mg g-1 CK和10.21mg g-1GMc。 H。

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