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Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies

机译:建立重生的MMV芯片技术:实现微生物组分析和其他迄今无法获得的技术

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Background With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel. It demonstrated the great potential of the MMV technology with the examples of 1024-parallel-cell culture and PCR experiments. However, its full potential failed to be expressed, owing to the nature of the material used for the MMV chip. Results In the present study, by developing plastic-based MMVs and associated technologies, we introduced novel technologies such as C2D2P (in which the cells in each well are converted from DNA to protein in 1024-parallel), NGS-non-dependent microbiome analysis, and other powerful applications. Conclusions The reborn MMV-microarray technology has proven to be highly efficient and cost-effective (with approximately 100-fold cost reduction) and enables us to realize hitherto unattainable technologies.
机译:背景技术随着生物科学的加速发展,研究成本问题变得越来越重要。我们以前使用软凝胶设计并开发了具有可控制体积(MMV)的新型概念微阵列。它通过1024个并行细胞培养和PCR实验实例证明了MMV技术的巨大潜力。但是,由于用于MMV芯片的材料的性质,未能充分发挥其潜力。结果在本研究中,通过开发基于塑料的MMV和相关技术,我们引入了新技术,例如C2D2P(其中每个孔中的细胞以1024平行的方式从DNA转化为蛋白质),NGS非依赖性微生物组分析,以及其他功能强大的应用程序。结论事实证明,重生的MMV芯片技术是高效且具有成本效益的(成本降低了约100倍),使我们能够实现迄今为止无法实现的技术。

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