• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Biotechnology >Nano-biolistics: a method of biolistic transfection of cells and tissues using a gene gun with novel nanometer-sized projectiles
【24h】

Nano-biolistics: a method of biolistic transfection of cells and tissues using a gene gun with novel nanometer-sized projectiles

机译：纳米生物弹药：一种使用带有新型纳米尺寸弹丸的基因枪对细胞和组织进行生物弹药转染的方法

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Biolistic transfection is proving an increasingly popular method of incorporating DNA or RNA into cells that are difficult to transfect using traditional methods. The technique routinely uses 'microparticles', which are ~1 μm diameter projectiles, fired into tissues using pressurised gas. These microparticles are efficient at delivering DNA into cells, but cannot efficiently transfect small cells and may cause significant tissue damage, thus limiting their potential usefulness. Here we describe the use of 40 nm diameter projectiles - nanoparticles - in biolistic transfections to determine if they are a suitable alternative to microparticles. Results Examination of transfection efficiencies in HEK293 cells, using a range of conditions including different DNA concentrations and different preparation procedures, reveals similar behaviour of microparticles and nanoparticles. The use of nanoparticles, however, resulted in ~30% fewer damaged HEK293 cells following transfection. Biolistic transfection of mouse ear tissue revealed similar depth penetration for the two types of particles, and also showed that 20% in microparticle-transfected samples. Visualising details of small cellular structures was also considerably enhanced when using nanoparticles. Conclusions We conclude that nanoparticles are as efficient for biolistic transfection as microparticles, and are more appropriate for use in small cells, when examining cellular structures and/or where tissue damage is a problem.
机译：背景技术射弹转染被证明是将DNA或RNA掺入难以使用传统方法转染的细胞中的一种越来越流行的方法。该技术通常使用直径约1μm的“微粒”，并使用加压气体将其发射到组织中。这些微粒可以有效地将DNA传递到细胞中，但不能有效地转染小细胞，并且可能引起明显的组织损伤，从而限制了它们的潜在用途。在这里，我们描述了在射弹转染中使用直径为40 nm的弹丸-纳米颗粒，以确定它们是否适合替代微粒。结果使用一系列条件（包括不同的DNA浓度和不同的制备程序）检查HEK293细胞中的转染效率，发现了微粒和纳米颗粒的相似行为。然而，纳米颗粒的使用导致转染后受损的HEK293细胞减少约30％。小鼠耳组织的弹射性转染显示出两种类型颗粒的相似深度渗透，并且还显示在微粒转染的样品中有20％的渗透。当使用纳米颗粒时，小细胞结构细节的可视化也大大增强。结论我们得出的结论是，当检查细胞结构和/或存在组织损伤的地方时，纳米粒子与微粒一样有效地用于生物弹射转染，并且更适合用于小型细胞。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号