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首页> 外文期刊>BMC Biotechnology >Optimization of culture conditions to produce high yields of active Acetobacter sp. CCTCC M209061 cells for anti-Prelog reduction of prochiral ketones
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Optimization of culture conditions to produce high yields of active Acetobacter sp. CCTCC M209061 cells for anti-Prelog reduction of prochiral ketones

机译：优化培养条件以产生高产的活性醋杆菌。 CCTCC M209061细胞可用于前手性酮的抗Prelog还原

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Background Chiral alcohols are widely used in the synthesis of chiral pharmaceuticals, flavors and functional materials and appropriate whole-cell biocatalysts offer a highly enantioselective, minimally polluting route to these valuable compounds. The recently isolated strain Acetobacter sp. CCTCC M209061 showed exclusive anti-Prelog stereoselectivity for the reduction of prochiral ketones, but the low biomass has limited its commercialization and industrial applications. To tackle this problem, the effects of medium components and culture conditions on the strain's growth and reduction activity were explored. Results By using a one-at-a-time method and a central composite rotatable design (CCRD), the optimal medium and culture conditions were found to be as follows: glucose 8.26 g/L, fructose 2.50 g/L, soy peptone 83.92 g/L, MnSO4·H2O 0.088 g/L, pH 5.70, 30°C and 10% (v/v) inoculum. Under the above-mentioned conditions, the biomass after 30 h cultivation reached 1.10 ± 0.03 g/L, which was 9.5-fold higher than that obtained with basic medium. Also, the reduction activity towards 4'-chloroacetophenone was markedly enhanced to 39.49 ± 0.96 μ mol/min/g from 29.34 ± 0.65 μ mol/min/g, with the product e.e . being above 99%. Comparable improvements were also seen with the enantioselective bioreduction of 4-(trimethylsilyl)-3-butyn-2-one to the key pharmaceutical precursor ( R ) - 4-(trimethylsilyl)-3-butyn-2-ol. Conclusions The biomass and reduction activity of Acetobacter sp. CCTCC M209061 can be greatly enhanced through the optimization strategy. This facilitates use of the strain in the anti-Prelog stereoselective reduction of prochiral ketones to enantiopure chiral alcohols as building blocks for many industries.
机译：背景技术手性醇被广泛用于手性药物，香料和功能材料的合成中，并且合适的全细胞生物催化剂为这些有价值的化合物提供了高度对映选择性，污染最小的途径。最近分离的菌株醋杆菌属。 CCTCC M209061对减少前手性酮具有独特的抗Prelog立体选择性，但低生物量限制了其商业化和工业应用。为了解决这个问题，研究了培养基成分和培养条件对菌株生长和还原活性的影响。结果采用一次一次性方法和中央复合可旋转设计（CCRD），发现最佳培养基和培养条件如下：葡萄糖8.26 g / L，果糖2.50 g / L，大豆蛋白83 83.92 g / L，MnSO 4 ·H 2 O 0.088 g / L，pH 5.70， 30°C和10％（v / v）接种物。在上述条件下，培养30h后的生物量达到1.10±0.03g / L，比碱性培养基高9.5倍。同样，对4′-氯苯乙酮的还原活性从29.34±0.65μmol/ min / g显着提高到39.49±0.96μmol/ min / g，产物为。高于99％。通过将4-（三甲基甲硅烷基）-3-丁炔-2-酮对映选择性生物还原为关键药物前体（R）-4-（三甲基甲硅烷基）-3-丁炔-2-醇也看到了类似的改进。结论醋杆菌（Acetobacter sp。）通过优化策略可以大大增强CCTCC M209061。这有利于将菌株用于前手性酮的抗Prelog立体选择性还原成对映纯手性醇，这是许多行业的基础。

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