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首页> 外文期刊>BMC Biotechnology >Echium acanthocarpum hairy root cultures, a suitable system for polyunsaturated fatty acid studies and production
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Echium acanthocarpum hairy root cultures, a suitable system for polyunsaturated fatty acid studies and production

机译：棘皮棘皮毛状根培养物，适用于多不饱和脂肪酸研究和生产的系统

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Background The therapeutic and health promoting role of highly unsaturated fatty acids (HUFAs) from fish, i.e. eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3) are well known. These same benefits may however be shared by some of their precursors, the polyunsaturated fatty acids (PUFAs), such as stearidonic acid (SDA, 18:4 n-3). In order to obtain alternative sources for the large-scale production of PUFAs, new searches are being conducted focusing on higher plants oils which can contain these n-3 and n-6 C18 precursors, i.e. SDA and GLA (18:3n-6, γ-linolenic acid). Results The establishment of the novel Echium acanthocarpum hairy root cultures represents a powerful tool in order to research the accumulation and metabolism of fatty acids (FAs) in a plant particularly rich in GLA and SDA. Furthermore, this study constitutes the first example of a Boraginaceae species hairy root induction and establishment for FA studies and production. The dominant PUFAs, 18:2n-6 (LA, linoleic acid) and 18:3n-6 (GLA), accounted for about 50% of total FAs obtained, while the n-3 PUFAs, 18:3n-3 (ALA, α-linolenic acid) and 18:4n-3 (SDA), represented approximately 5% of the total. Production of FAs did not parallel hairy root growth, and the optimal productivity was always associated with the highest biomass density during the culture period. Assuming a compromise between FA production and hairy root biomass, it was determined that sampling times 4 and 5 gave the most useful FA yields. Total lipid amounts were in general comparable between the different hairy root lines (29.75 and 60.95 mg/g DW), with the major lipid classes being triacylglycerols. The FAs were chiefly stored in the hairy roots with very minute amounts being released into the liquid nutrient medium. Conclusions The novel results presented here show the utility and high potential of E. acanthocarpum hairy roots. They are capable of biosynthesizing and accumulating a large range of polyunsaturated FAs, including the target GLA and SDA fatty acids in appreciable quantities.
机译：背景技术鱼中的高度不饱和脂肪酸（HUFA），即二十碳五烯酸（EPA，20：5n-3）和二十二碳六烯酸（DHA，22：6n-3）的治疗和健康促进作用是众所周知的。但是，它们的某些前体（多不饱和脂肪酸（PUFA），例如硬脂酸（SDA，18：4 n-3））也可以共享这些相同的好处。为了获得大规模生产PUFA的替代来源，目前正在针对可能含有这些n-3和n-6 C18前体即SDA和GLA（18：3n-6， γ-亚麻酸）。结果建立新的棘皮棘毛根培养物是研究富含GLA和SDA的植物中脂肪酸（FAs）积累和代谢的有力工具。此外，该研究构成了紫草科物种毛状根诱导和建立FA研究和生产的第一个实例。占主导地位的PUFA为18：2n-6（LA，亚油酸）和18：3n-6（GLA），约占所获FA的50％，而n-3 PUFA为18：3n-3（ALA， α-亚麻酸）和18：4n-3（SDA）约占总数的5％。 FA的产生与毛状根的生长不平行，并且最佳生产力始终与培养期间的最高生物量密度相关。假设FA产量和根状毛生物量之间存在折衷，可以确定采样时间4和5给出了最有用的FA产量。总的脂质量在不同的毛状根系（29.75和60.95 mg / g DW）之间通常是可比的，主要的脂质类别是三酰基甘油。 FAs主要储存在毛状根中，极少量被释放到液态营养培养基中。结论此处提出的新结果显示了刺五加毛状根的实用性和高潜力。它们能够生物合成和积累大量的多不饱和脂肪酸，包括相当数量的目标GLA和SDA脂肪酸。

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