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首页> 外文期刊>BMC Biotechnology >Repair of large segmental bone defects: BMP-2 gene activated muscle grafts vs. autologous bone grafting
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Repair of large segmental bone defects: BMP-2 gene activated muscle grafts vs. autologous bone grafting

机译：大节段性骨缺损的修复：BMP-2基因激活的肌肉移植与自体骨移植

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Background Common cell based strategies for the treatment of osseous defects require the isolation and expansion of autologous cells. Since this makes such approaches time-consuming and expensive, we developed a novel expedited technology creating gene activated muscle grafts. We have previously shown that large segmental bone defects in rats can be regenerated by implantation of muscle tissue fragments activated by BMP-2 gene transfer. Results In the present study, we compared the bone healing capacities of such gene activated muscle grafts with bone isografts, mimicking autologous bone grafting, the clinical gold standard for treatment of bone defects in patients. Two of 14 male, syngeneic Fischer 344 rats used for this experiment served as donors for muscle and bone. Muscle tissue was harvested from both hind limbs and incubated with an adenoviral vector carrying the cDNA encoding BMP-2. Bone was harvested from the iliac crest and long bone epiphyses. Bone defects (5?mm) were created in the right femora of 12 rats and were filled with either BMP-2 activated muscle tissue or bone grafts. After eight weeks, femora were evaluated by radiographs, micro-computed tomography (μCT), and biomechanical testing. In the group receiving BMP-2 activated muscle grafts as well as in the bone-grafting group, 100% of the bone defects were healed, as documented by radiographs and μCT-imaging. Bone volume was similar in both groups and biomechanical stability of the two groups was statistically indistinguishable. Conclusions This study demonstrates that treatment of large bone defects by implantation of BMP-2 gene activated muscle tissue leads to similar bone volume and stability as bone isografts, mimicking autologous bone grafting.
机译：背景技术用于治疗骨缺损的基于普通细胞的策略要求自体细胞的分离和扩增。由于这使此类方法既耗时又昂贵，因此我们开发了一种新颖的加速技术来创建基因激活的肌肉移植物。先前我们已经表明，可以通过植入由BMP-2基因转移激活的肌肉组织片段来再生大鼠的大型节段性骨缺损。结果在本研究中，我们将这种基因激活的肌肉移植物与骨同种异体移植物（模拟自体骨移植物，这是治疗患者骨缺损的临床金标准）的骨愈合能力进行了比较。用于该实验的14只雄性同种Fischer 344大鼠中的两只用作肌肉和骨骼的供体。从两个后肢收获肌肉组织，并与携带编码BMP-2的cDNA的腺病毒载体一起温育。从the和长骨骨phy中收获骨头。在12只大鼠的右股骨中形成骨缺损（5？mm），并用BMP-2活化的肌肉组织或骨移植物填充。八周后，通过射线照相，微型计算机断层扫描（μCT）和生物力学测试评估股骨。放射线照相和μCT成像显示，在接受BMP-2活化的肌肉移植物的组以及植骨组中，100％的骨缺损得到了治愈。两组的骨体积相似，两组的生物力学稳定性在统计学上没有区别。结论这项研究表明，通过植入BMP-2基因激活的肌肉组织来治疗大骨缺损，其骨体积和稳定性与同种异体骨相似，可模仿自体骨移植。

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