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首页> 外文期刊>BMC Biotechnology >Decolorization applicability of sol–gel matrix immobilized manganese peroxidase produced from an indigenous white rot fungal strain Ganoderma lucidum
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Decolorization applicability of sol–gel matrix immobilized manganese peroxidase produced from an indigenous white rot fungal strain Ganoderma lucidum

机译：本地白腐真菌灵芝产生的溶胶-凝胶基质固定化的锰过氧化物酶的脱色适用性

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Background An eco-friendly treatment of industrial effluents is a major environmental concern of the modern world in the face of stringent environmental legislations. By keeping in mind the extensive industrial applications of ligninolytic enzymes, this study was performed to purify, and immobilize the manganese peroxidase (MnP) produced from an indigenous strain of Ganoderma lucidum . The present study was also focused on investigating the capability of immobilized MnP for decolorization of dye containing textile effluents. Results A large magnitude of an indigenous MnP (882±13.3 U/mL) was obtained from white rot fungal strain G . lucidum in solid state bio-processing of wheat straw under optimized fermentation conditions (moisture, 50%; substrate, 5 g; pH, 5.5; temperature, 30°C; carbon source, 2% fructose; nitrogen source, 0.02% yeast extract; C: N ratio, 25:1; fungal spore suspension, 5 mL and fermentation time period, 4 days). After ammonium sulfate fractionation and Sephadex -G-100 gel filtration chromatography, MnP was 4.7-fold purified with specific activity of 892.9 U/mg. G . lucidum MnP was monomeric protein as evident by single band corresponding to 48 kDa on native and denaturing SDS-PAGE . The purified MnP (2 mg/mL) was immobilized using a sol–gel matrix of tetramethoxysilane (TMOS) and proplytrimethoxysilane (PTMS). The oxidation of MnSO4 for up to 10 uninterrupted cycles demonstrated the stability and reusability of the immobilized MnP. Shelf life profile revealed that enzyme may be stored for up to 60 days at 25°C without losing much of its activity. To explore the industrial applicability of MnP produced by G . lucidum , the immobilized MnP was tested against different textile effluents. After 4 h reaction time, the industrial effluents were decolorized to different extents (with a maximum of 99.2%). The maximally decolorized effluent was analyzed for formaldehyde and nitroamines and results showed that the toxicity parameters were below the permissible limits. Conclusions In conclusion, G . lucidum MnP was immobilized by sol–gel matrix entrapment with an objective to enhance its practical efficiencies. The MnP was successfully entrapped into a sol- gel matrix of TMOS and PTMS with an overall immobilization efficiency of 93.7%. The sol- gel entrapped MnP seems to have prospective capabilities which can be useful for industrial purposes, especially for bioremediation of industrial effluents.
机译：背景技术面对严格的环境法规，对工业废水进行生态友好的处理是现代世界关注的主要环境问题。考虑到木质素分解酶的广泛工业应用，进行了本研究以纯化和固定由灵芝本地菌株生产的锰过氧化物酶（MnP）。本研究还专注于研究固定化MnP对含染料的纺织品废水进行脱色的能力。结果从白腐真菌菌株G中获得了大量的原生MnP（882±13.3 U / mL）。在优化的发酵条件下（水分为50％;底物为5 g; pH为5.5;温度为30°C;碳源为2％果糖;氮源为0.02％酵母提取物;固态氮为5％）; C∶N比为25∶1;真菌孢子悬浮液为5mL，发酵时间为4天。经硫酸铵分级分离和Sephadex -G-100凝胶过滤色谱分离后，MnP纯化4.7倍，比活性为892.9 U / mg。 G 。透明质酸MnP是单体蛋白，通过在天然和变性SDS-PAGE上对应于48 kDa的单条带证实。使用四甲氧基硅烷（TMOS）和丙三甲氧基硅烷（PTMS）的溶胶-凝胶基质固定纯化的MnP（2 mg / mL）。 MnSO 4 的氧化过程长达10个不间断循环，证明了固定化MnP的稳定性和可重复使用性。保质期显示，酶可以在25°C下最多保存60天，而不会失去很多活性。探讨G生产的MnP的工业适用性。灵芝，针对不同的纺织品废水测试了固定化的MnP。反应4小时后，工业废水的脱色程度不同（最大为99.2％）。分析了最大脱色的废水中的甲醛和硝基胺，结果表明毒性参数低于允许的限值。结论总之，G。透明质酸MnP通过溶胶-凝胶基质包裹法固定化，目的是提高其实际效率。 MnP被成功捕获到TMOS和PTMS的溶胶-凝胶基质中，总固定效率为93.7％。溶胶-凝胶包裹的MnP似乎具有前瞻性功能，可用于工业目的，尤其是用于工业废水的生物修复。

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