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首页> 外文期刊>BMC Biotechnology >Identification of an endo-1,4-beta-xylanase of Ustilago maydis
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Identification of an endo-1,4-beta-xylanase of Ustilago maydis

机译：鉴定Ustilago maydis的1,4-β-木聚糖内切酶

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Background The utilization of raw biomass components such as cellulose or hemicellulose for the production of valuable chemicals has attracted considerable research interest in recent years. One promising approach is the application of microorganisms that naturally convert biomass constituents into value added chemicals. One of these organisms – Ustilago maydis – can grow on xylan, the second most abundant polysaccharide in nature, while at the same time it produces chemicals of biotechnological interest. Results In this study, we present the identification of an endo-1,4-beta xylanase responsible for xylan degradation. Xylanase activity of U . maydis cells was indirectly detected by the quantification of released reducing sugars and could be confirmed by visualizing oligosaccharides as degradation products of xylan by thin layer chromatography. A putative endo-1,4-beta-xylanase, encoded by um06350.1, was identified in the supernatant of xylan-grown cells. To confirm the activity, we displayed the putative xylanase on the surface of the xylanase negative Saccharomyces cerevisiae EBY100. The presented enzyme converted xylan to xylotriose, similar to the source organism U . maydis . Conclusions The xylan degradation ability together with its unicellular and yeast-like growth makes U. maydis MB215 a promising candidate for the production of valuable chemicals such as itaconic acid or glycolipids from lignocellulosic biomass. Therefore, the characterization of the endo-1,4-beta-xylanase, encoded by um06350.1, is a further step towards the biotechnological application of U . maydis and its enzymes.
机译：背景技术近年来，诸如纤维素或半纤维素的原始生物质组分用于生产有价值的化学物质已经引起了相当大的研究兴趣。一种有前途的方法是应用微生物，该微生物自然地将生物质成分转化为增值化学品。其中一种生物体-乌斯季亚哥（Ustilago maydis）-可以在木聚糖上生长，木聚糖是自然界中含量第二高的多糖，同时还可以产生对生物技术感兴趣的化学物质。结果在这项研究中，我们提出了一种负责木聚糖降解的内切1，4-β木聚糖酶的鉴定。 U的木聚糖酶活性。 maydis细胞通过定量还原释放的糖间接检测到，并可以通过薄层色谱观察低聚糖作为木聚糖的降解产物来确认。在木聚糖生长的细胞的上清液中鉴定出由um06350.1编码的推定的1,4-β-木聚糖内切酶。为了证实活性，我们在木聚糖酶阴性酿酒酵母EBY100的表面上展示了推定的木聚糖酶。呈现的酶将木聚糖转化为木三糖，类似于来源生物U。 Maydis。结论木聚糖降解能力以及其单细胞和类酵母的生长能力使美里氏菌MB215成为从木质纤维素生物质生产有价值的化学物质（如衣康酸或糖脂）的有前途的候选者。因此，表征由um06350.1编码的1，4-β-木聚糖内切酶是朝着U的生物技术应用迈出的又一步。 maydis及其酶。

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