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首页> 外文期刊>Biotechnology for Biofuels >Moderate alkali-thermophilic ethanologenesis by locally isolated Bacillus licheniformis from Pakistan employing sugarcane bagasse: a comparative aspect of aseptic and non-aseptic fermentations
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Moderate alkali-thermophilic ethanologenesis by locally isolated Bacillus licheniformis from Pakistan employing sugarcane bagasse: a comparative aspect of aseptic and non-aseptic fermentations

机译：来自巴基斯坦的局部分离的地衣芽孢杆菌利用甘蔗渣进行中度碱温热乙醇生成：无菌和非无菌发酵的比较方面

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BackgroundBiofuels obtained from first-generation (1G) sugars-starch streams have been proven unsustainable as their constant consumption is not only significantly costly for commercial scale production systems, but it could potentially lead to problems associated with extortionate food items for human usage. In this regard, biofuels’ production in alkali-thermophilic environs from second-generation (2G) bio-waste would not only be markedly feasible, but these extreme conditions might be able to sustain aseptic fermentations without spending much for sterilization. ResultsPresent investigation deals with the valuation of ethanologenic potential of locally isolated moderate alkali-thermophilic fermentative bacterium, Bacillus licheniformis KU886221 employing sugarcane cane bagasse (SCB) as substrate. A standard 2-factor central composite response surface design was used to estimate the optimized cellulolytic and hemicellulolytic enzymatic hydrolysis of SCB into maximum fermentable sugars. After elucidation of optimized levels of fermentation factors affecting ethanol fermentation using Taguchi OA L27 (3^13) experimental design, free cell batch culture was carried out in bench-scale stirred-tank bioreactor for ethanol fermentation. Succeeding fermentation modifications included subsequent substrate addition, immobilized cells fibrous-bed bioreactor (FBB) incorporation to the basic setup, and performance of in situ gas stripping for attaining improved ethanol yield. Highest ethanol yield of 1.1406?mol ethanol/mol of equivalent sugars consumed was obtained when gas stripping was performed during fed-batch fermentation involving FBB under aseptic conditions. Despite the fact that under non-aseptic conditions, 30.5% lesser ethanol was formed, still, reduced yield might be considered influential as it saved the cost of sterilization for ethanol production. ConclusionEffectual utilization of low-priced abundantly available lignocellulosic waste sugarcane bagasse under non-aseptic moderate alkali-thermophilic fermentation conditions as directed in this study has appeared very promising for large-scale cost-effective bioethanol generation processes.
机译：背景技术从第一代（1G）糖淀粉流中获得的生物燃料已被证明是不可持续的，因为它们的持续消耗不仅对于商业规模的生产系统而言成本高昂，而且还可能导致与人类使用勒索性食品相关的问题。在这方面，由第二代（2G）生物废料在碱性嗜温环境中生产生物燃料不仅非常可行，而且这些极端条件可能能够维持无菌发酵而无需花费大量杀菌费用。结果目前的研究涉及以甘蔗蔗渣（SCB）为底物的本地分离的中等碱温性发酵细菌KU886221产乙醇潜力的评估。标准的2因子中央复合响应表面设计用于评估将SCB最佳化为最大可发酵糖的纤维素水解和半纤维素酶水解。在使用Taguchi OA L27（3 ^ 13）实验设计阐明了影响乙醇发酵的最佳发酵因子水平后，在台式规模的搅拌罐式生物反应器中进行了无细胞分批培养，以进行乙醇发酵。成功的发酵修饰包括随后的底物添加，固定化的细胞纤维床生物反应器（FBB）并入基本设置，以及原位汽提以提高乙醇产量的性能。当在无菌条件下，在涉及FBB的分批补料发酵过程中进行气提时，乙醇的最高产量为1.1406？mol乙醇/ mol当量糖。尽管在非无菌条件下形成的乙醇减少了30.5％，但仍可以认为收率降低是有影响的，因为它节省了生产乙醇的灭菌成本。结论如本研究所述，在非无菌中度碱温热发酵条件下有效利用低价，丰富的木质纤维素废甘蔗渣对大规模生产具有成本效益的生物乙醇工艺来说，是很有希望的。

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