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首页> 外文期刊>BMC Biotechnology >Expression and functional analyses of a Kinesin gene GhKIS13A1 from cotton (Gossypium hirsutum) fiber
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Expression and functional analyses of a Kinesin gene GhKIS13A1 from cotton (Gossypium hirsutum) fiber

机译：棉（棉）纤维驱动蛋白基因GhKIS13A1的表达及功能分析

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BackgroundCotton fiber, a natural fiber widely used in the textile industry, is differentiated from single cell of ovule epidermis. A large number of genes are believed to be involved in fiber formation, but so far only a few fiber genes have been isolated and functionally characterized in this developmental process. The Kinesin13 subfamily was found to play key roles during cell division and cell elongation, and was considered to be involved in the regulation of cotton fiber development. ResultsThe full length of coding sequence of GhKIS13A1 was cloned using cDNA from cotton fiber for functional characterization. Expression pattern analysis showed that GhKIS13A1 maintained a lower expression level during cotton fiber development. Biochemical assay showed that GhKIS13A1 has microtubule binding activity and basal ATPase activity that can be activated significantly by the presence of microtubules. Overexpression of GhKIS13A1 in Arabidopsis reduced leaf trichomes and the percentage of three-branch trichomes, and increased two-branch and shriveled trichomes compared to wild-type. Additionally, the expression of GhKIS13A1 in the Arabidopsis Kinesin-13a-1 mutant rescued the defective trichome branching pattern of the mutant, making its overall trichome branching pattern back to normal. ConclusionsOur results suggested that GhKIS13A1 is functionally compatible with AtKinesin-13A regarding their role in regulating the number and branching pattern of leaf trichomes. Given the developmental similarities between cotton fibers and Arabidopsis trichomes, it is speculated that GhKIS13A1 may also be involved in the regulation of cotton fiber development.
机译：背景棉纤维是纺织工业中广泛使用的天然纤维，与胚珠表皮的单细胞有所区别。人们相信大量基因参与了纤维的形成，但是到目前为止，只有少数的纤维基因被分离出来并在此发育过程中进行了功能鉴定。发现Kinesin13亚家族在细胞分裂和细胞伸长过程中起关键作用，并被认为参与棉纤维发育的调控。结果利用棉纤维cDNA克隆了GhKIS13A1的全长编码序列，进行了功能鉴定。表达模式分析表明，GhKIS13A1在棉纤维发育过程中保持较低的表达水平。生化分析表明，GhKIS13A1具有微管结合活性和基础ATPase活性，这些活性可以被微管的存在显着激活。与野生型相比，拟南芥中GhKIS13A1的过表达减少了叶片毛状体和三分支毛状体的百分比，并增加了两个分支和枯萎的毛状体。此外，GhKIS13A1在拟南芥Kinesin-13a-1突变体中的表达挽救了该突变体的缺陷毛状体分支模式，使其总体毛状体分支模式恢复正常。结论我们的结果表明，GhKIS13A1在调节叶片毛状体的数量和分支模式方面与AtKinesin-13A在功能上兼容。考虑到棉纤维和拟南芥的毛状体之间的发育相似性，推测GhKIS13A1也可能参与棉纤维发育的调控。

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