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首页> 外文期刊>BMC Medical Genomics >Serum microRNAs profile from genome-wide serves as a fingerprint for diagnosis of acute myocardial infarction and angina pectoris
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Serum microRNAs profile from genome-wide serves as a fingerprint for diagnosis of acute myocardial infarction and angina pectoris

机译：来自全基因组的血清microRNAs图谱可作为诊断急性心肌梗塞和心绞痛的指纹图谱

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Background In order to identify miRNAs expression profiling from genome-wide screen for diagnosis of acute myocardial infarction (AMI) and angina pectoris (AP), we investigated the altered profile of serum microRNAs in AMI and AP patients at a relative early stage. Methods Serum samples were taken from 117 AMI patients, 182 AP patients and 100 age-and gender-matched controls. An initial screening of miRNAs expression was performed by Solexa sequencing. Differential expression was validated using RT-qPCR in individuals samples, the samples were arranged in a two-phase selection and validation. Results The Solexa sequencing results demonstrated marked upregulation of serum miRNAs in AMI patients compared with controls. RT-qPCR analysis identified a profile of six serum miRNAs (miR-1, miR-134, miR-186, miR-208, miR-223 and miR-499) as AMI biomarkers. MiR-208 and miR-499 were elevated higher in AP cases than in AMI cases. The ROC curves indicated a panel of six miRNAs has a great potential to offer sensitive and specific diagnostic tests for AMI. More especially, the panel of six miRNAs presents significantly differences between the AMI and AP cases. Conclusions The six-miRNAs signature identified from genome-wide serum miRNA expression profiling may serves as a fingerprint for AMI and AP diagnosis.
机译：背景技术为了从全基因组筛选中鉴定miRNA表达谱，以诊断急性心肌梗塞（AMI）和心绞痛（AP），我们在相对早期研究了AMI和AP患者血清microRNA的变化。方法从117例AMI患者，182例AP患者和100例年龄和性别相匹配的对照组中抽取血清。通过Solexa测序进行miRNA表达的初步筛选。使用RT-qPCR验证个体样品中的差异表达，将样品分为两阶段进行选择和验证。结果Solexa测序结果表明，与对照组相比，AMI患者血清miRNA明显上调。 RT-qPCR分析鉴定了六种血清miRNA（miR-1，miR-134，miR-186，miR-208，miR-223和miR-499）的谱作为AMI生物标志物。 AP患者的MiR-208和miR-499水平高于AMI患者。 ROC曲线表明，一组六个miRNA具有很大的潜力，可以为AMI提供灵敏且特异的诊断测试。更具体地说，六个miRNA的组在AMI和AP病例之间存在显着差异。结论从全基因组血清miRNA表达谱中鉴定出的6个miRNA特征可作为AMI和AP诊断的指纹。

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