• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Ophthalmology >Different alpha crystallin expression in human age-related and congenital cataract lens epithelium
【24h】

Different alpha crystallin expression in human age-related and congenital cataract lens epithelium

机译：人类年龄相关性和先天性白内障晶状体上皮中不同的alpha crystallin表达

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background The purpose of this study was to investigate the different expressions of αA-crystallin and αB-crystallin in human lens epithelium of age-related and congenital cataracts. Methods The central part of the human anterior lens capsule approximately 5?mm in diameter together with the adhering epithelial cells, were harvested and processed within 6?hours after cataract surgery from age-related and congenital cataract patients or from normal eyes of fresh cadavers. The mRNA and soluble protein levels of αA-crystallin and αB-crystallin in the human lens epithelium were detected by real-time PCR and western blots, respectively. Results The mRNA and soluble protein expressions of αA-crystallin and αB-crystallin in the lens epithelium were both reduced in age-related and congenital cataract groups when compared with the normal control group. However, the degree of α-crystallin loss in the lens epithelium was highly correlated with different cataract types. The α-crystallin expression of the lens epithelium was greatly reduced in the congenital cataract group but only moderately decreased in the age-related cataract group. The reduction of αA-crystallin soluble protein levels in the congenital cataract group was approximately 2.4 fold decrease compared with that of the age-related cataract group, while an mRNA fold change of 1.67 decrease was observed for the age-related cataract group. Similarly, the reduction of soluble protein levels of αB-crystallin in the congenital cataract group was approximately a 1.57 fold change compared with that of the age-related cataract group. A 1.75 fold change for mRNA levels compared with that of the age-related cataract group was observed. Conclusions The results suggest that the differential loss of α-crystallin in the human lens epithelium could be associated with the different mechanisms of cataractogenesis in age-related versus congenital cataracts, subsequently resulting in different clinical presentations.
机译：背景研究的目的是研究年龄相关性和先天性白内障的晶状体上皮中αA-晶状体蛋白和αB-晶状体蛋白的不同表达。方法在年龄和先天性白内障患者或新鲜尸体的正常眼中，在白内障手术后6小时内，收集并处理直径约5？mm的人前晶状体囊的中心部分及其粘附的上皮细胞。实时荧光定量PCR和蛋白质印迹分别检测人晶状体上皮中αA-晶状蛋白和αB-晶状蛋白的mRNA和可溶性蛋白水平。结果与正常对照组相比，年龄相关性和先天性白内障组晶状体上皮中αA-晶状体蛋白和αB-晶状体蛋白的mRNA和可溶性蛋白表达均降低。然而，晶状体上皮中α-晶状蛋白的损失程度与不同的白内障类型高度相关。先天性白内障组晶状体上皮的α-晶状体蛋白表达大大降低，而与年龄相关的白内障组仅中等程度降低。与年龄相关性白内障组相比，先天性白内障组中αA-晶状体蛋白可溶性蛋白水平降低约2.4倍，而年龄相关性白内障组观察到mRNA倍数变化为1.67。类似地，与年龄相关性白内障组相比，先天性白内障组中αB-晶状体蛋白的可溶性蛋白水平降低约1.57倍。与年龄相关性白内障组相比，mRNA水平变化了1.75倍。结论结果表明，人晶状体上皮中α-晶状体蛋白的差异损失可能与年龄相关性白内障与先天性白内障的白内障发生机制不同有关，从而导致不同的临床表现。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号