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首页> 外文期刊>BMC nutrition. >Hypolipidemic effect and activation of Lecithin Cholesterol Acyl Transferase (LCAT) by aqueous extract of Spirulina platensis during toxicological investigation
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Hypolipidemic effect and activation of Lecithin Cholesterol Acyl Transferase (LCAT) by aqueous extract of Spirulina platensis during toxicological investigation

机译：螺旋藻水提取物的降血脂作用和卵磷脂胆固醇酰基转移酶的激活作用

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Background Spirulina platensis produced in Nomayos (Cameroon) is used as a dietary supplement. S. platensis is known as a neutraceutical with many beneficial effects on humans like lipid-lowering action. This study aims to investigate the mechanism of hypolipidemic action of aqueous extract of Spirulina platensis ( S. platensis ) through the toxicological studies. MethodsIn this study, we included two month old Wistar rats, weighing between 180 and 200?g. Aqueous S. platensis was extracted and prepared using standard methods. The rats received a supplementation of S. platensis at 5000?mg/Kg of body weight as single dose in acute toxicity whereas different doses (250, 500, 1000?mg / kg body weight) were administered in subacute toxicity compared to control. Acute and subacute toxicities were determined according to the guidelines 420 (14?days) and 407 (28?days) of the Organization for Economic Cooperation and Development (OECD) respectively. Biochemical parameters such as urea, creatinine, total and direct bilirubin, lipid profile and transaminases; and histopathological analysis of the liver and kidneys were used to evaluate the toxicity of S. platensis on these Wistar rats. Plasmatic hydroxymethyl glutaryl coenzyme A reductase (HMG CoA reductase) and lecithine cholesterol acyl transferase (LCAT) were performed to explain the lipid-lowering action of S. platensis . Histopathological analysis of the liver and kidneys was performed. ResultsOur results show a decrease in total cholesterol for male rats (from 84 to 74?mg/dl) when the dose of S. platensis increased; this reduction of the total cholesterol level in male rats was significant at 500?mg/kg. There was also a significant inhibition of HMG CoA reductase in a dose dependent manner between 25 and 84.5 fold compared to the control in both male and female groups. At the dose of 250?mg/kg bw, the level of LCAT was higher compared with other groups and control, but the difference was not statistically significant. A slight inflammation in the liver and the mesangial hyperplasia of the renal glomeruli was revealed by the histopathological investigation in subacute toxicity . Conclusion Spirulina platensis from Cameroon appears to have little toxic effects and may demonstrate hypolipidemic activity through the activation of LCAT.
机译：背景技术在Nomayos（喀麦隆）生产的螺旋藻被用作膳食补充剂。 S. platensis被称为营养保健品，对人类具有许多有益的作用，例如降脂作用。本研究旨在通过毒理学研究螺旋藻水提物的降血脂作用机理。方法在这项研究中，我们纳入了两个月大的Wistar大鼠，体重在180至200微克之间。用标准方法提取和制备水链金葡菌。大鼠在急性毒性下以单剂量5000？mg / Kg补充了链球菌，而与对照组相比，在亚急性毒性下给予了不同剂量（250、500、1000？mg / kg体重）。急性和亚急性毒性分别根据经济合作与发展组织（OECD）的准则420（14天）和407（28天）确定。生化参数，例如尿素，肌酐，总胆红素和直接胆红素，脂质分布和转氨酶;并通过肝脏和肾脏的组织病理学分析来评估钝顶链球菌对这些Wistar大鼠的毒性。进行了血浆羟甲基戊二酰辅酶A还原酶（HMG CoA还原酶）和卵磷脂胆固醇酰基转移酶（LCAT）的研究，以解释S. platensis的降脂作用。进行了肝脏和肾脏的组织病理学分析。结果我们的结果表明，当高原鼠兔的剂量增加时，雄性大鼠的总胆固醇降低（从84毫克/分升至74？mg / dl）;雄性大鼠总胆固醇水平的这种降低在500？mg / kg时是显着的。在男性和女性组中，与对照组相比，HMG CoA还原酶的剂量依赖性均显着抑制25至84.5倍。剂量为250？mg / kg bw时，LCAT的水平高于其他组和对照组，但差异无统计学意义。通过亚急性毒性的组织病理学研究发现肝脏轻度炎症和肾小球系膜增生。结论喀麦隆的螺旋藻几乎没有毒性作用，可能通过激活LCAT表现出降血脂活性。

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