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首页> 外文期刊>BMC research notes >Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves
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Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves

机译：白花蛇舌草叶片总酚含量和体外抗氧化活性的估算

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Background Research on natural products has gained a wide popularity due to the potential of discovering active compounds . The antioxidant properties contained in plants have been proposed as one of the mechanisms for the observed beneficial effect. Therefore, the present study investigated the antioxidant activity and total phenolic contents of various solvent extracts of Albizia procera leaves. Methods Antioxidant activity of the methanol extract and its derived fractions petroleum ether (APP), carbon tetrachloride (APC), dichloromethane (APD), ethyl acetate (APE), and residual aqueous fraction (APA) of the leaves of Albizia procera was performed by in vitro chemical analyses. Total phenolic content of the APM and other five fractions were also determined. APM and its derived fractions were also subjected to preliminary phytochemical screening test for various constituents. R esults Phytochemical screening revealed the presence of saponins, steroids, tannins, glycosides and flavonoids in the extracts. Amongst the extracts, APE showed the highest total phenolic content (449.18?±?18.41mg of gallic acid equivalent/g of extract). In DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging test, the IC50 value of APM, APP, APC, APD, APE and APA was 43.43, 63.60, 166.18, 41.15, 11.79, and 63.06 μg/mL, respectively. Therefore, among the APM and its derived fractions, APE showed the highest antioxidant activity which is comparable to that of standard ascorbic acid (AA) (IC50 10.12 μg/mL). The total antioxidant capacity was found to be varied in different fractions. The reducing activity on ferrous ion was ranked as APE?>?APD?>?APM?>?APA?>?APC. C onclusion The above evidences suggest that APE of A. procera leaf is a potential source of natural antioxidant and can be used to prevent diseases associated with free radicals.
机译：背景天然产物的研究由于发现活性化合物的潜力而获得了广泛的普及。已经提出了植物中所含的抗氧化剂特性是观察到的有益效果的机制之一。因此，本研究调查了苦艾叶的各种溶剂提取物的抗氧化活性和总酚含量。方法采用HPLC法对Albizia procera叶片的甲醇提取物及其衍生组分石油醚（APP），四氯化碳（APC），二氯甲烷（APD），乙酸乙酯（APE）和残留水组分（APA）进行抗氧化作用。体外化学分析。还确定了APM和其他五个馏分的总酚含量。 APM及其衍生馏分也经过了各种成分的初步植物化学筛选测试。结果植物化学筛选显示提取物中存在皂苷，类固醇，单宁，糖苷和类黄酮。在提取物中，APE的总酚含量最高（每克提取物中的没食子酸当量为449.18？±？18.41mg）。在DPPH（1,1-二苯基-2-picylhydrazyl）自由基清除测试中，APM，APP，APC，APD，APE和APA的IC 50 值为分别为43.43、63.60、166.18、41.15、11.79和63.06μg/ mL。因此，在APM及其衍生馏分中，APE的抗氧化活性最高，可与标准抗坏血酸（AA）（IC 50 10.12μg/毫升）。发现总的抗氧化剂能力在不同部分中变化。对亚铁离子的还原活性为APE→> APD→>→APM→>→APA→>→APC。结论上述证据表明，proc。procera叶的APE是天然抗氧化剂的潜在来源，可用于预防与自由基有关的疾病。

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