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首页> 外文期刊>BMC research notes >In vitro inhibition of human leukemia THP-1 cells by Origanum syriacum L. and Thymus vulgaris L. extracts
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In vitro inhibition of human leukemia THP-1 cells by Origanum syriacum L. and Thymus vulgaris L. extracts

机译：牛至和百里香提取物对人白血病THP-1细胞的体外抑制作用

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Background Natural products including, traditional medicinal plants have emerged as a tempting alternative to conventional chemotherapeutic protocols of leukemia because of their minimum side effects and less documented drug resistance. Methods Ethanol extracts were prepared from Thymus vulgaris L. and Origanum syriacum L. plants and investigated against the THP-1 leukemia cell line and freshly isolated peripheral blood mononuclear cells (PBMCs). The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay and the lactate dehydrogenase (LDH) assay were respectively used to determine the cellular viability and cytotoxicity in response to treatment with increasing extract concentrations. Results Both extracts exhibited a concentration dependent reduction in viability of the THP-1 cells (IC50?=?2.126?mg/mL for O. syriacum , and 0.1569?mg/mL for T. vulgaris ). O. syriacum was more potent against the PBMCs (IC50?=?0.4247?mg/mL), while T. vulgaris was moderately selective (IC50?=?0.3345?mg/mL with PBMCs and SI?=?2.1). Only in O. syriacum the reduction in cells viability was caused by cytotoxic effect against leukemic cells (LC50?=?of 9.646?mg/mL). Conclusion T. vulgaris and O. syriacum are both antileukemic in vitro. T. vulgaris represents a potential selective cytostatic and safe target for future anticancer agents’ development. O. syriacum on the other hand is cytotoxic against the leukemia cell line THP-1.
机译：背景技术包括传统药用植物在内的天然产品已成为白血病常规化疗方案的诱人替代品，因为它们的副作用极小，耐药性也较低。方法从百里香和牛至的植物中提取乙醇提取物，并针对THP-1白血病细胞系和新鲜分离的外周血单个核细胞（PBMC）进行研究。分别使用3- [4,5-二甲基噻唑-2-基] -2,5-二苯基溴化四氮唑（MTT）测定法和乳酸脱氢酶（LDH）测定法来确定随着治疗剂量的增加，细胞活力和细胞毒性提取物浓度。结果两种提取物均显示出浓度依赖性地降低了THP-1细胞的活力（对于柳丁鱼，IC 50 α=？2.126？mg / mL，而对于寻常斑潜蝇，其浓度为0.1569？mg / mL）。 。 syriacum对PBMC有更强的效用（IC 50 α=？0.4247？mg / mL），而寻常T. vulgaris具有中等选择性（IC 50 α=？0.3345 PBMC和SI？=？2.1的？mg / mL）。仅在稻曲霉中，细胞活力的降低是由对白血病细胞的细胞毒性作用引起的（LC 50 α=？为9.646？mg / mL）。结论寻常锥虫和鼠伤寒沙门氏菌均在体外具有抗白血病作用。寻常型锥虫是未来抗癌药发展的潜在选择性细胞抑制和安全靶标。另一方面，O。syriacum对白血病细胞系THP-1具有细胞毒性。

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