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首页> 外文期刊>BMC Biochemistry >Mechano-growth factor accelerates the proliferation and osteogenic differentiation of rabbit mesenchymal stem cells through the PI3K/AKT pathway
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Mechano-growth factor accelerates the proliferation and osteogenic differentiation of rabbit mesenchymal stem cells through the PI3K/AKT pathway

机译：机械生长因子通过PI3K / AKT途径促进兔间充质干细胞的增殖和成骨分化

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摘要

Mesenchymal stem cells (MSCs) can differentiate into chondroblasts, adipocytes, or osteoblasts under appropriate stimulation. Mechano-growth factor (MGF) reportedly displays a neuroprotective effect in cerebral regions that were exposed to ischemia and is expressed in stromal cells of the eutopic endometrium and in glandular cells of the ectopic endometrium. This study sought to understand the potential involvement of phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT) in MGF-induced growth of rabbit MSCs (rMSCs). We applied various concentrations of MGF to cultured rMSCs and observed the growth rate of the cells, the changes in the phosphorylation state of AKT and mammalian target of rapamycin (mTOR), and the expression levels of alkaline phosphatase and osteocalcin. We found that the growth and osteogenic differentiation of MGF-induced rMSCs were promoted primarily by phosphorylated AKT, and that this phosphorylation, as well mTOR phosphorylation, was mediated by the MGF receptor. Our study suggests that MGF promotes the growth and osteogenic differentiation of rMSCs primarily through the PI3K/AKT pathway.
机译：在适当的刺激下，间充质干细胞（MSC）可以分化为成软骨细胞，脂肪细胞或成骨细胞。据报道，机械生长因子（MGF）在暴露于局部缺血的脑区域中表现出神经保护作用，并在异位内膜的间质细胞和异位内膜的腺细胞中表达。这项研究试图了解磷脂酰肌醇3-激酶（PI3K）/蛋白激酶B（AKT）在MGF诱导的兔MSC（rMSCs）生长中的潜在作用。我们将各种浓度的MGF应用于培养的rMSCs，观察细胞的生长速率，AKT磷酸化状态的变化和雷帕霉素（mTOR）的哺乳动物靶标，以及碱性磷酸酶和骨钙素的表达水平。我们发现，MGF诱导的rMSCs的生长和成骨分化主要是由磷酸化的AKT促进的，并且该磷酸化以及mTOR磷酸化是由MGF受体介导的。我们的研究表明，MGF主要通过PI3K / AKT途径促进rMSC的生长和成骨分化。

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