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首页> 外文期刊>Bosnian Journal of Basic Medical Sciences >Huaiqihuang may protect from proteinuria by resisting MPC5 podocyte damage via targeting p-ERK/CHOP pathway
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Huaiqihuang may protect from proteinuria by resisting MPC5 podocyte damage via targeting p-ERK/CHOP pathway

机译：怀七黄可能通过靶向p-ERK / CHOP途径抵抗MPC5足细胞损伤，从而预防蛋白尿

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The purpose of this study was to investigate the potential effects of Huaiqihuang (HQH) granule, a Chinese herbal medicine, in treating proteinuria and to reveal its possible mechanism. MPC5 podocytes were cultured in vitro at 37°C and induced with tunicamycin (TM). The TM-induced cells were treated with HQH at different concentrations. The cell proliferation was detected using the MTT assay. The optimal effective dose of HQH for MPC5 cells was determined by the MTT assay and LDH assay respectively. The influences of HQH on the proteinuria-related protein expression and the signaling pathway associated protein expression were also detected using quantitative reverse transcription PCR and Western blotting analysis. The results showed that the MPC5 cell model was successfully constructed in vitro . The HQH application could improve the harmful effects induced by TM on the MPC5 cells, including promoted cell proliferation and suppressed cell apoptosis. Furthermore, the protein expression, including podocin, nephrin, and synaptopodin was down-regulated by the TM treatment in the MPC5 cells. On contrary, the expression of these proteins was up-regulated after the HQH application. Also, the effect of TM on integrin α3 and integrin β1 expressions was also reversed by the HQH treatment. Moreover, the HQH application decreased the expression of p-ERK and DNA-damage-inducible transcript 3 (DDIT3 or CHOP) in the MPC5 cells, which was opposite to the effect observed in the cells treated with TM. Taken together, our study suggest that HQH application may protect podocytes from TM damage by suppressing the p-ERK/CHOP signaling pathway.
机译：这项研究的目的是调查中药怀芪黄（HQH）颗粒在治疗蛋白尿中的潜在作用并揭示其可能的机制。 MPC5足细胞在37°C体外培养，并用衣霉素（TM）诱导。 TM诱导的细胞用不同浓度的HQH处理。使用MTT测定法检测细胞增殖。分别通过MTT法和LDH法确定对MPC5细胞HQH的最佳有效剂量。还使用定量逆转录PCR和Western印迹分析检测了HQH对蛋白尿相关蛋白表达和信号通路相关蛋白表达的影响。结果表明，成功构建了MPC5细胞模型。 HQH的应用可以改善TM对MPC5细胞的有害作用，包括促进细胞增殖和抑制细胞凋亡。此外，通过TM处理，MPC5细胞中的蛋白质表达（包括podocin，nephrin和synaptopodin）被下调。相反，在HQH应用后，这些蛋白质的表达上调。同样，HQH处理也逆转了TM对整联蛋白α3和整联蛋白β1表达的作用。此外，HQH的施用降低了MPC5细胞中p-ERK的表达和DNA损伤诱导性转录本3（DDIT3或CHOP）的表达，这与在TM处理的细胞中观察到的作用相反。两者合计，我们的研究表明HQH的应用可能通过抑制p-ERK / CHOP信号通路来保护足细胞免受TM损伤。

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