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首页> 外文期刊>Breeding science >Agronomic traits and gene containment capability of cleistogamous rice lines with the superwoman1-cleistogamy mutation
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Agronomic traits and gene containment capability of cleistogamous rice lines with the superwoman1-cleistogamy mutation

机译：superwoman1-cleoogamy突变的cleosogamous水稻品系的农艺性状和基因容纳能力

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摘要

Pollen-mediated transgene flow is a major concern for the production of genetically modified (GM) rice. Cleistogamy is a useful tool for preventing this form of gene flow. We previously identified the cleistogamous rice mutant superwoman1-cleistogamy ( spw1-cls ) and determined its molecular genetic mechanism. In the present study, we cultivated spw1-cls over five years to examine effects of cleistogamy on agronomic traits. Simultaneously, we cultivated cleistogamous backcross lines created by continuous backcrossing with “Yumeaoba” (a japonica cultivar) as the recurrent parent and by application of a DNA marker. In these experimental cultivations, spw1-cls and its backcross lines showed almost equal or slightly lower, but acceptable, agronomic traits compared with each control line. We also conducted natural crossing tests in paddy fields to assess the gene containment capability of spw1-cls . In a series of field experiments, there was no natural crossing between spw1-cls (pollen donor) and pollen recipient lines, but the wild-type donor and recipient lines were crossed. Thus, the cleistogamy of the spw1-cls mutation is able to inhibit natural crossing effectively, without significant loss of commercial benefits, such as yield. We conclude that spw1-cls cleistogamy is a practical tool for gene containment in GM rice cultivation.
机译：花粉介导的转基因流是转基因水稻生产的主要问题。 Cleistogamy是防止这种形式的基因流动的有用工具。我们以前确定了杂种水稻突变体superwoman1-cleistogamy（spw1-cls）并确定了其分子遗传机制。在本研究中，我们在5年中培养了spw1-cls，以研究变态配偶对农艺性状的影响。同时，我们培养了通过以轮回亲本“ Yumeaoba”（粳稻品种）进行连续回交并应用DNA标记而产生的交配回交系。在这些实验性栽培中，与每个对照品系相比，spw1-cls及其回交品系显示出几乎相等或略低但可接受的农艺性状。我们还在稻田中进行了自然杂交试验，以评估spw1-cls的基因容纳能力。在一系列的田间实验中，spw1-cls（花粉供体）和花粉受体系之间没有自然杂交，但野生型供体和受体系杂交了。因此，spw1-cls突变的变态反应能有效抑制自然杂交，而不会显着损失商业利益，例如产量。我们得出的结论是，spw1-cls配伍是在转基因水稻栽培中进行基因遏制的实用工具。

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