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Isolation of Legionella Species from Noyu (Unattended Natural Hot Springs in Mountains and Fields) Samples in Japan

机译:日本野汤(I> Noyu )中军团菌的分离

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In order to understand the habitation conditions of the bacteria of the genus Legionella in Noyu (unattended natural hot springs in mountains and fields) in Japan, isolation of Legionella spp. was attempted in the Noyu samples from 11 prefectures nationwide between May and September 2012, and the following results were obtained. Overall, Legionella spp. was isolated from 16 of 43 samples (37.2%). The species was isolated from the Hokkaido region to the Chugoku region but not from the Shikoku region to the Kyushu region. The number of bacteria detected was usually small, less than 5.0×10~(1) CFU/100 ml, as found in 11 samples (68.8%), while counts of 10~(2) or more to 10~(3) or less CFU/100 ml were found in two samples (12.5%). Legionella pneumophila was the most commonly found strain, with 19 strains (90.5%) found, and was the dominant species. Regarding the serogrouping, four strains (21.1%) fell under group 1, the most common grouping, followed by three strains (15.8%) in group 3, two strains (10.5%) in group 5, etc. Moreover, the detected bacterial strains other than L. pneumophila included two strains (9.5%) of L. londiniensis . The temperature of the Noyu from which Legionella spp. was isolated was between 33.1°C and 41.5°C with a pH ranging from 5.2 to 8.1. The present report is the first report to clarify the habitation conditions of strains of Legionella spp. isolated from Noyu in Japan.
机译:为了了解日本的 Noyu(山区和田野无人看管的天然温泉)中 Legionella菌的细菌的栖息条件,请分离 Legionella spp。在2012年5月至2012年9月期间,从全国11个州的Noyu样本中尝试了这种方法,并获得了以下结果。总体而言,Legionella spp。从43个样本中分离出16个(37.2%)。该物种是从北海道地区到中国地区,但不是从四国地区到九州地区。在11个样本中发现的细菌数量通常很少,小于5.0×10〜(1)CFU / 100 ml(68.8%),而计数在10〜(2)以上至10〜(3)或以上的细菌数量为10在两个样品中发现的CFU / 100 ml更少(12.5%)。 嗜肺军团菌是最常见的菌株,共发现19株(占90.5%),是优势种。在血清分组方面,第1组为最常见的4个菌株(21.1%),第3组为3个菌株(15.8%),第5组为2个菌株(10.5%),以此类推。除了 L。肺炎杆菌包括两株(9.5%)的L。 londiniensis。 Legionella spp的 Noyu的温度。分离的产物在33.1℃至41.5℃之间,pH为5.2至8.1。本报告是第一份阐明Legionella spp菌株的居住条件的报告。与日本的野汤分离。

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