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首页> 外文期刊>Biocontrol Science >Enhanced Colonization of rpoS-Deficient Escherichia coli Cells on Solid Surfaces by Reinforced csgA Gene Expression
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Enhanced Colonization of rpoS-Deficient Escherichia coli Cells on Solid Surfaces by Reinforced csgA Gene Expression

机译：增强 csgA 基因表达增强 rpoS 缺陷型大肠杆菌细胞在固体表面的定殖

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Scanning electron microscopy revealed that the rpoS -deficient cells of E. coli K-12 BW25113 (ΔrpoS ) increased the number of flagella on the cell surfaces. However, the quantitative analysis of cell colonization showed that the increased number of flagella on ΔrpoS cell surfaces did not cause the enhancement of cell colonization on the surfaces of polyvinyl chloride (PVC), polypropylene (PP) and polystyrene (PS) after 24 h of incubation at 37℃. To facilitate the enhanced expression of curli, the csgA gene was introduced into the ΔrpoS cells. The transformed cells rich in flagella and curli on the cell surfaces were found to make colonies 2-3 times larger than both the wild type and ΔrpoS cells on the PVC, PP and PS surfaces at 37℃. It was thus verified that the reinforcement of csgA gene in the ΔrpoS cells induced the enhanced colonization on the solid surfaces with the increased flagellum and curli expressions.
机译：扫描电子显微镜揭示出 E的 rpoS缺陷细胞。大肠杆菌K-12 BW25113（ΔrpoS）增加了细胞表面鞭毛的数量。然而，细胞定殖的定量分析表明，ΔrpoS细胞表面上鞭毛数目的增加并未引起聚氯乙烯（PVC），聚丙烯（PP）和聚苯乙烯（PS）表面细胞定殖的增强。在37℃孵育24小时后。为了促进curli的增强表达，将csgA基因引入ΔrpoS细胞。在37℃下，发现在细胞表面上富含鞭毛和卷曲的转化细胞的集落比在PVC，PP和PS表面上的野生型和ΔrpoS细胞大2-3倍。因此证实了在ΔrpoS细胞中的csgA基因的增强诱导了固体表面上定殖的增强，鞭毛和卷曲的表达增加了。

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