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Isolation and Identification of Enterobacteriaceae from Raw Horsemeat intended for Human Consumption (Basashi)

机译：用于人类消费的生马肉中的肠杆菌科的分离和鉴定（Basashi）

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The status of Enterobacteriaceae contamination was investigated in a total of 131 samples of raw horsemeat (Basashi) intended for human consumption purchased from a general meat shop or by mail-order from October 2012 to December 2013. The bacteria were isolated from 105 of the 112 samples (93.8%). Prominent differences in the isolation rate due to the place of manufacture/sale or by the cut of the meat were not observed. Moreover, in a comparison between domestic (92.6%) and imported (100%) samples, the isolation rate was slightly higher in the imported samples.When Enterobacteriaceae isolated from raw horsemeat was identified, it was highly diverse, with 14 species identified in total. From among these species, Hafnia alvei was the most common, with 33 strains (19.8%),followed by 27 strains (16.2%) of Klebsiella pneumoniae and 26 strains (15.6%) of Enterobacter cloacae , indicating that these three species were dominant. A trend was observed, with the dominant strain differing depending on the place of manufacture/sale or the cut of the meat. H. alvei was isolated at an especially high frequency from imported samples.An investigation was carried out regarding raw horsemeat intended for human consumption from Yamanashi Prefecture and Canada, regularly purchased from one store in Kanagawa Prefecture. Enterobacteriaceae were isolated during five of nine (55.6%） trials, in which the isolated bacteria were H. alvei, K. pneumoniae , etc. Moreover, they were isolated at a very high isolation rate of seven among 10 trials for the Canadian meat, and H. alvei was the most commonly isolated bacteria. Accordingly, when an investigation was carried out regarding the differences in the strain level in the six isolates of H. alvei periodically isolated from raw horsemeat from Canada by the pulsed-field gel electrophoresis (PFGE) pattern using a restriction enzyme, SfiI , there was a possibility that these were the same H-38 strain (November 2013) and H-64 strain (April 2014) as well as the same H-104 strain (July 2014) and H-131 strain (December 2014).As mentioned above, it has been demonstrated that a variety of Enterobacteriaceae were isolated from raw horsemeat (Basashi) intended for human consumption, and at a high frequency. Moreover, based on the fact that the same species or strain was chronologically isolated, the possibility of contamination by the same contamination source at different times was suggested.
机译：在2012年10月至2013年12月期间，从普通肉店或通过邮购购买的供人类食用的生马肉（Basashi）共有131个样品，调查了肠杆菌科细菌的污染状况。该细菌是从105个细菌中分离出来的112个样本中（93.8％）。没有观察到由于生产/销售地点或切肉造成的隔离率显着差异。此外，在对国内样品（92.6％）和进口样品（100％）进行比较时，进口样品的分离率略高。当鉴定出从生马肉中分离出的肠杆菌科细菌时，其高度多样性，有14种总共确定。在这些物种中，Hafnia alvei最常见，有33株（19.8％），其次是肺炎克雷伯菌27株（16.2％）和泄殖腔肠杆菌26株（15.6％），表明这三个物种占优势。观察到一种趋势，主要应变随制造/销售地点或肉块的不同而不同。 H。 alvei从进口样品中的分离频率特别高。对山梨县和加拿大拟供人类食用的生马肉进行了调查，这些生马肉通常从神奈川县的一家商店购买。在9项试验（55.6％）中，有5项分离了肠杆菌科细菌，其中分离的细菌是H. alvei，肺炎克雷伯氏菌等。此外，在10项试验中有7项的分离率很高在加拿大的肉类中进行试验，而H. alvei是最常分离的细菌，因此，当对定期从生食中分离出的六个H. alvei分离株的菌株水平进行调查时，通过使用限制酶SfiI的脉冲场凝胶电泳（PFGE）模式从加拿大获得的马肉，它们可能是同一H-38菌株（2013年11月）和H-64菌株（2014年4月）以及相同的H-104菌株（2014年7月）和H-131菌株（2014年12月）。如上所述，已证明从用于人类的生马肉（Basashi）中分离出多种肠杆菌科且频率很高。此外，基于相同的规格es或菌株按时间顺序隔离，建议在同一时间由同一污染源污染的可能性。

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