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首页> 外文期刊>Biocontrol Science >Biosynthesis of Low Molecular Weight Antifungal Protein from Aspergillus giganteus in Batch Fermentation and In-Vitro Assay.
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Biosynthesis of Low Molecular Weight Antifungal Protein from Aspergillus giganteus in Batch Fermentation and In-Vitro Assay.

机译：分批发酵和体外测定法从大曲霉中合成低分子量抗真菌蛋白。

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In present study, Taguchi's design of experiment L_(9)orthogonal array was created using Qualitek-4 software with four most critical factors namely, K_(2)HPO_(4), MgSO_(4), CaCl_(2)and culture pH. Production of a new intracellular antifungal protein in submerged fermentation was optimized with yield of 0.98±0.1 mg/gram dry cell weight mycelia from Aspergillus giganteus MTCC 8408. The average molecular mass of the purified protein was figured as 5.122 kDa using Electro Spray Ionization-Mass Spectrometry. Scanning electron microscopy was used to correlate the effect of selected factors on fungal cell morphology and its metabolite production. In vitro antifungal susceptibility assay was profiled against Aspergillus niger and minimum inhibitory concentrations were in the range 0.3±0.06 μg/ml. The stronger influencing factors on afp production and mycelial biomass were noted with CaCl_(2)and K_(2)HPO_(4)respectively. The validation experiments using optimized conditions confirmed an improvement in afp by 3.86 times with mycelial biomass by 1.52 times, compared to the basal medium. The present statistical optimization study revealed an opportunity to promote economical design at the industrial level for future scale up of effective antifungal agent against systemic aspergillosis as well as possible post harvest loss.
机译：在本研究中，Taguchi使用Qualitek-4软件创建了实验L_（9）正交阵列的设计，该软件具有四个最关键的因素，即K_（2）HPO_（4），MgSO_（4），CaCl_（2）和培养pH。优化了深层发酵中新的细胞内抗真菌蛋白的生产，其产自米曲霉MTCC 8408的干细胞菌丝体的产量为0.98±0.1 mg / g。使用电喷雾电离质谱法，纯化的蛋白的平均分子量为5.122 kDa。光谱法。扫描电子显微镜用于关联所选因素对真菌细胞形态及其代谢产物的影响。针对黑曲霉进行了体外抗真菌药敏试验，最小抑菌浓度为0.3±0.06μg/ ml。 CaCl_（2）和K_（2）HPO_（4）分别显示了对afp产生和菌丝生物量的更强影响因子。使用优化条件的验证实验证实，与基础培养基相比，菌丝体生物量的afp提高了3.86倍，是1.52倍。当前的统计优化研究表明，有机会在工业水平上进行经济的设计，以便将来扩大针对系统性曲霉病以及可能造成收获后损失的有效抗真菌剂的规模。

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