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Comparison of Chromogenic Selective Media for the Detection of Cronobacter spp. (Enterobacter sakazakii).

机译:用于检测克氏杆菌的生色选择性培养基的比较。 (阪崎肠杆菌)。

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The four types of chromogenic selective media that are commercially available in Japan were compared for establishing a Japanese standard method for detecting Cronobacter spp. based on ISO/TS 22964:2006. When assessed using 9 standard Cronobacter spp. strains and 29 non-Cronobacter strains, Enterobacter sakazakii isolation agar, Chromocult~(TM)Enterobacter sakazakii agar, CHROMagar~(TM)E. sakazakii, and XM-sakazakii agar demonstrated excellent inclusivity and exclusivity. Using the ISO/TS 22964:2006 method, the recovered numbers of 38 Cronobacter spp. strains, including 29 C. sakazakii isolates obtained from each medium, were equivalent, indicating that there was no significant difference (p > 0.05) among the four types of chromogenic selective media. Thus, we demonstrated that these four chromogenic selective media are suitable alternatives when using the standard method for detecting Cronobacter spp. in Japan, based on the ISO/TS 22964:2006.
机译:比较了在日本可商购的四种生色选择培养基,以建立日本标准检测克氏杆菌的方法。基于ISO / TS 22964:2006。使用9个标准Cronobacter spp评估时。菌株和29种非克罗诺杆菌菌株,阪崎肠杆菌分离琼脂,ChromocultTM阪崎肠杆菌琼脂,CHROMagarTME。 sakazakii和XM-sakazakii琼脂表现出出色的包容性和排他性。使用ISO / TS 22964:2006方法,回收到38个克氏杆菌属物种。菌株,包括从每种培养基中获得的29株阪崎肠杆菌分离株,是等效的,表明在四种类型的生色选择性培养基之间没有显着差异(p> 0.05)。因此,我们证明了使用标准方法检测克氏杆菌属时,这四种生色选择性培养基是合适的替代品。在日本基于ISO / TS 22964:2006。

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