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首页> 外文期刊>British Biotechnology Journal >In vitro Direct Regeneration of Cotton (Gossypium spp.) Cultivars of Sudan
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In vitro Direct Regeneration of Cotton (Gossypium spp.) Cultivars of Sudan

机译：苏丹棉品种的体外直接再生

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In vitro direct plantlets’ development from different explants of cotton was studied using nine cultivars which have been developed in Sudan. The influence of various phytohormones on morphogenetic response of different explants of each cultivar was tested. The best medium for multiple shoot induction from shoot tip explants was Murashige and Skoog (MS) basal medium supplemented with 0.1 mg/L Zeatin. In this medium, the highest regeneration percentage (92%) and the maximum number of shoots (2) were recorded for Sudac K cultivar. Shoot elongation and rooting of most of the shooted explants were obtained in 0.05 mg/L Gibberellic acid (GA3)-supplemented MS medium. Embryo axis explants were best regenerated in MS medium supplemented with 0.1 mg/L Kinetin + 0.1 mg/ L 2,4-D in seven of the nine tested cultivars. The highest shooting percentage (80%) was recorded for Shambat-B followed by Hamid cultivar (75%). The majority of the shoots (62.4%) showed root formation in MS medium supplemented with Kinetin + 2,4-D medium. Non- rooted shoots derived from shoot tip and embryo axis explants showed a range of 90-100 rooting percentages when transferred into 0.1 mg/ L IBA- supplemented MS medium. Cotyledonary nodes explants of cultivars Abdin, Kheiralla, Barakat-90, Knight and Barakat were found to form shoots in 0.1 mg/L Kinetin-supplemented MS medium, with the highest percentage (79%) recorded for Barakat cultivar. Rooting was induced, for all shooted explants, in ? strength MS medium supplemented with 0.1 mg/L NAA.
机译：利用苏丹开发的9个栽培品种，研究了不同棉花外植体对体外直接小植株的发育。测试了各种植物激素对每个品种不同外植体形态发生反应的影响。从芽尖外植体中诱导多芽的最佳培养基是补充0.1 mg / L玉米素的Murashige和Skoog（MS）基础培养基。在该培养基中，记录了Sudac K品种的最高再生百分比（92％）和最大芽数（2）。在添加0.05 mg / L赤霉素（GA 3 ）的MS培养基中，获得了大多数芽苗外植体的芽伸长和生根。在添加了0.1 mg / L激动素+ 0.1 mg / L 2,4-D的MS培养基中，在9个测试品种中有7个使胚轴外植体最佳再生。 Shambat-B的射击率最高（80％），其次是Hamid品种（75％）。大多数芽（62.4％）在补充了Kinetin + 2,4-D培养基的MS培养基中显示出根形成。当从根尖和胚轴外植体衍生的无根芽转移到0.1 mg / L IBA补充的MS培养基中时，显示出90-100的生根百分比。发现在0.1 mg / L补充Kinetin的MS培养基中，品种Abdin，Kheiralla，Barakat-90，Knight和Barakat的子叶节外植体形成了芽，其中，Barakat品种的记录百分比最高（79％）。对于所有射出的外植体，在？强度的MS​​培养基，添加0.1 mg / L NAA。

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