...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Microbiology >Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in Vibrio cholerae
【24h】

Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in Vibrio cholerae

机译：双精氨酸易位系统对霍乱弧菌生物膜形成，定殖和毒力的多效性影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background The Twin-arginine translocation (Tat) system serves to translocate folded proteins, including periplasmic enzymes that bind redox cofactors in bacteria. The Tat system is also a determinant of virulence in some pathogenic bacteria, related to pleiotropic effects including growth, motility, and the secretion of some virulent factors. The contribution of the Tat pathway to Vibrio cholerae has not been explored. Here we investigated the functionality of the Tat system in V. cholerae, the etiologic agent of cholera. Results In V. cholerae, the tatABC genes function in the translocation of TMAO reductase. Deletion of the tatABC genes led to a significant decrease in biofilm formation, the ability to attach to HT-29 cells, and the ability to colonize suckling mouse intestines. In addition, we observed a reduction in the output of cholera toxin, which may be due to the decreased transcription level of the toxin gene in tatABC mutants, suggesting an indirect effect of the mutation on toxin production. No obvious differences in flagellum biosynthesis and motility were found between the tatABC mutant and the parental strain, showing a variable effect of Tat in different bacteria. Conclusion The Tat system contributes to the survival of V. cholerae in the environment and in vivo, and it may be associated with its virulence.
机译：背景技术双精氨酸转运（Tat）系统用于转运折叠的蛋白质，包括结合细菌中氧化还原辅因子的周质酶。 Tat系统还是某些致病菌中毒力的决定因素，与多效性作用有关，包括生长，运动和某些毒性因子的分泌。 Tat途径对霍乱弧菌的贡献尚未探索。在这里，我们研究了霍乱病原体霍乱弧菌中Tat系统的功能。结果在霍乱弧菌中，tatABC基因在TMAO还原酶的易位中起作用。 tatABC基因的删除导致生物膜形成，附着于HT-29细胞的能力以及定居在乳鼠肠道中的能力显着下降。此外，我们观察到霍乱毒素的输出减少，这可能是由于tatABC突变体中毒素基因的转录水平降低所致，表明该突变对毒素产生具有间接影响。在tatABC突变体和亲本菌株之间，鞭毛的生物合成和活力没有明显差异，表明Tat在不同细菌中的作用不同。结论Tat系统有助于霍乱弧菌在环境和体内的存活，并可能与其毒性有关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号