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首页> 外文期刊>British Biotechnology Journal >Isolation and Identification of Methicillin-Resistant Staphylococcus aureus from Keys ofCollege Students Using Different DetectionMethods
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Isolation and Identification of Methicillin-Resistant Staphylococcus aureus from Keys ofCollege Students Using Different DetectionMethods

机译：不同检测方法从大学生重点人群中分离鉴定耐甲氧西林金黄色葡萄球菌

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Aims: In this study, the methicillin-resistant Staphylococcus aureus (MRSA) were isolated and identified by using biochemical tests, antibiogram and polymerase chain reaction (PCR) to explore the circulation of MRSA among college students. Study design: Cross-sectional study.Place and Duration of Study: Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia between June 2010 and December 2010.Methodology: A total of 100 samples were collected from keys of college students. There were 39 isolates (39 %) Gram-positive cocci and Catalase positive. 29 (74.36%) were glucose oxidation and fermentation positive. From the 39 isolates, 16 (43.24%) were shown Mannitol Salt Agar (MSA) tests positive. The deoxyribonuclease (DNase) tests and tube coagulase tests with human and rabbit plasma were carried out to improve the efficiency of the MSA test.Results: 7 (43.75%) DNase positive and 2 (12.5%) tube coagulase positive. Both human and rabbit plasma showed similar sensitivity for the tube coagulase tests in this study. However, both isolates with tube coagulase positive were confirmed as S. aureus but not resistant to oxacillin, methicillin, erythromycin and cefoxitin. 2 (66.67%) of 3 (18.75%) isolates which is tube coagulase negative were resistant to erythromycin and 1 (33.33%) of them was resistant to methicillin. Rare strains of S. aureus can be coagulase negative. PCR assay was used. 1 (33.33%) of the coagulase negative isolate resistant to erythromycin was found to have nuc gene, mecA gene, ermC gene, msrA gene, linA gene, and femA gene. The isolate was confirmed as MRSA.Conclusion: In conclusion, PCR technique is more sensitive and reliable than tube coagulase test or antibiogram for the detection of MRSA. And keys were shown to be an important source of MRSA and other bacteria circulation in the community.
机译：目的：本研究通过生化测试，抗菌素谱图和聚合酶链反应（PCR）分离和鉴定耐甲氧西林金黄色葡萄球菌（MRSA），探索大学生中MRSA的循环。研究设计：横断面研究研究地点和持续时间：2010年6月至2010年12月在马来西亚雪兰莪雪兰莪大学（University Putra）于43400 UPM Serdang的马来西亚普特拉大学医学微生物学系。方法：总共100样本是从大学生的关键中收集的。有39株（39 ％）革兰氏阳性球菌和过氧化氢酶阳性。葡萄糖氧化和发酵呈阳性的占29（74.36 ％）。从39个分离物中，有16个（43.24％）的甘露醇盐琼脂（MSA）测试呈阳性。进行了人和兔血浆的脱氧核糖核酸酶（DNase）检测和管凝结酶检测，以提高MSA检测的效率。结果：DNase阳性7例（43.75％），管凝凝酶2例（12.5 %%）。在这项研究中，人血浆和兔血浆对管凝血酶测试均显示出相似的敏感性。然而，两种具有管凝固酶阳性的分离株均被确认为金黄色葡萄球菌，但对奥沙西林，甲氧西林，红霉素和头孢西丁没有耐药性。 3株（18.75％）的管凝结酶阴性菌株中有2株（66.67％）对红霉素有抗性，其中1株（33.33％）对甲氧西林有抗性。金黄色葡萄球菌的罕见菌株可能是凝固酶阴性的。使用PCR测定法。发现对红霉素有抗性的凝固酶阴性分离株中有1个（33.33％）具有nuc基因，mecA基因，ermC基因，msrA基因，linA基因和femA基因。结论：结论：PCR技术比管凝固酶检测法或抗菌素检测法更灵敏，更可靠。并且密钥被证明是社区中MRSA和其他细菌循环的重要来源。

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