• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>British Biotechnology Journal >Treatment of Wastewaters Generated bySurfactant-Enhanced Washing of Soils in anAerobic Biofilter Inoculated with a Consortiumof Hydrocarbon Degraders
【24h】

Treatment of Wastewaters Generated bySurfactant-Enhanced Washing of Soils in anAerobic Biofilter Inoculated with a Consortiumof Hydrocarbon Degraders

机译：在由烃降解剂联合接种的需氧生物滤池中，通过表面活性剂-增强洗涤液处理产生的废水

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Aims: The aims of this work were 1) To evaluate the performance of a submerged biofiltration system for the treatment of a surfactant-enriched wastewater that had been generated by a soil washing process.2) To evaluate the effect of the flux and organic load over the performance of the system. 3) To determine the microbial evolution as an effect of the flux at different lengths of the biofilter by using a denaturing gradient gel electrophoresis (DGGE) analysis.Study Design: A three factorial design was used to evaluate the effect of different fluxes and organic loads over the performance of a continuously operated submerged aerobic biofilter. The DGGE technique was employed to determine microbial changes in the biofilter.Place and Duration of Study: The study was carried out at the Bioprocess Laboratory, Bioprocesses Department UPIBI-IPN, Mexico. The experimental stage lasted approximately eight months and the DGGE analysis four months more.Methodology: Contaminated soil was physicochemical and microbiologically characterized. A total of 70 kg of contaminated soil was washed using a 1:3 ratio soil/surfactant solution (0.5% Sulfopon 30-SP30). The surfactant-enriched wastewater was then treated in a submerged biofilter. The biofiltration system consisted of a column with a length of 50 cm and diameter of 12 cm. The biofilter was packed with tezontle with an average diameter of 0.2-0.4 cm and 70% void space. The biofilter working volume was 4.5 L. The samples of the packing material for the DGGE analysis were obtained from the ports located along the biofilter: at the wastewater inlet, at the middle of the column and at the outlet. After DNA extraction with a Power Soil DNA Isolation Kit (MO BIO), PCR (polymerase chain reaction) analysis was conducted. The 16S rRNA gene was amplified using universal bacterial primers. The data obtained by DGGE analysis for the microbial population developed in the biofilter were further analyzed by the Jaccard similarity coefficient.Results: The soil contained 14,704 mg/kg TPH. BTEX compounds were not found, and only two different PAHs were found in the soil samples: benzo-fluoranthene and benzopyrene, at concentrations of 0.1280 and 0.0682 mg/kg of soil, respectively. During the surfactant-aided soil washing, the highest removal percentage of the oil removed from the soil was 59% with 0.5% SP30. The wastewater generated after the soil washing process contained, in average 1,329 mg COD/L and 211 mg/L of grease and oil. Higher COD removals were obtained at a flux of 0.4 L/h for both of the COD initial concentrations. While the highest removal was 78.27%, determined at an initial COD concentration of 300 mg/L. When applying fluxes of 0.28 and 0.40 L/h at a higher initial COD concentration, the COD removals were increased; this was not the case for a flux of 0.63 L/h. For a given initial COD concentration, the removal efficiencies were higher for lower fluxes. Analysis of the similarity between the microbial populations for varying fluxes and levels along the length of the biofilter was determined by the Jaccard (JI) index. The results showed that the initial microbial populations (t0) have low similarities with the developed microbial populations at the different conditions tested.Conclusion: Both the flux and the initial COD concentration had an impact on COD removal and the microbial concentration in the column. The COD removal percentages were similar at fluxes of 0.28 and 0.63 L/h. The highest removal percentage of 78.27% was obtained at a flux of 0.4L/h; this finding was in agreement with the highest microbial count and the specialization of microbial populations (less diversity). In general, it was shown that the flux had an effect on changes in microbial population. Greater effects were observed on the microbial population due to the position along the reactor, e.g., the greatest differences were found at the different levels of the biofilter.
机译：目的：这项工作的目的是：1）评估淹没式生物过滤系统对处理土壤洗涤过程中产生的富含表面活性剂的废水的性能。2）评估通量和有机负荷的影响系统的性能。 3）通过变性梯度凝胶电泳（DGGE）分析确定微生物在不同长度的生物滤池中作为通量的影响研究设计：采用三因子设计来评估不同通量和有机负荷的影响连续运行的潜水式好氧生物滤池的性能。 DGGE技术用于确定生物滤池中的微生物变化。研究的地点和持续时间：该研究在墨西哥UPIBI-IPN生物过程部门的生物过程实验室进行。实验阶段持续了大约8个月，而DGGE分析又持续了4个月。方法：对被污染的土壤进行了物理化学和微生物学表征。使用比例为1：3的土壤/表面活性剂溶液（0.5％的Sulfopon 30-SP30）洗涤总共70 kg的污染土壤。然后，将富含表面活性剂的废水在浸没式生物滤池中进行处理。生物过滤系统由长度为50 cm，直径为12 cm的色谱柱组成。该生物过滤器装有平均直径为0.2-0.4厘米的Tezontle和70％的空隙空间。生物滤池的工作体积为4.5L。用于DGGE分析的填料的样品是从生物滤池的以下端口获得的：废水入口，色谱柱中部和出口。用Power Soil DNA分离试剂盒（MO BIO）提取DNA后，进行PCR（聚合酶链反应）分析。使用通用细菌引物扩增16S rRNA基因。通过Jaccard相似系数进一步分析了通过DGGE分析获得的生物滤池中微生物种群的数据。结果：土壤中TPH含量为14,704 mg / kg。没有发现BTEX化合物，在土壤样品中仅发现了两种不同的PAH：苯并荧蒽和苯并re，其浓度分别为0.1280和0.0682 mg / kg。在表面活性剂辅助的土壤洗涤过程中，用0.5％的SP30从土壤中去除油的最高去除率为59％。土壤洗涤后产生的废水平均含有1,329 mg COD / L和211 mg / L油脂。对于两种COD初始浓度，通量均为0.4 L / h时，可以获得更高的COD去除率。在初始COD浓度为300 mg / L时，最高去除率为78.27％。当在较高的初始COD浓度下使用0.28和0.40 L / h的通量时，COD的去除量会增加。通量为0.63 L / h的情况并非如此。对于给定的初始COD浓度，通量越低，去除效率就越高。通过Jaccard（JI）指数确定了沿生物滤池长度变化的通量和水平的微生物种群之间的相似性分析。结果表明，在不同的测试条件下，初始微生物种群（t0）与已发展的微生物种群的相似性较低。结论：通量和初始COD浓度都对COD去除和色谱柱中微生物浓度有影响。通量为0.28和0.63 L / h时，COD去除率相似。通量为0.4L / h时，去除率最高，为78.27％。这一发现与最高的微生物数量和微生物种群的专业化（多样性较低）相一致。通常，已表明通量对微生物种群的变化有影响。由于沿着反应器的位置，对微生物种群产生了更大的影响，例如，在生物过滤器的不同水平上发现了最大的差异。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号