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Evaluation of the Quantitative Dry Culture Method (SanitakunTM SA) for the Enumeration of Staphylococcus aureus in Artificially Contaminated Food Samples

机译:定量干燥培养方法(Sanitakun TM SA)计数人工污染食品样品中金黄色葡萄球菌的评价

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Sanita-kun~(TM) SA for Staphylococcus aureus (SkSA), a novel dry sheet quantitative culture system, was evaluated. When the inclusivity and exclusivity of SkSA were assessed using 121 microorganisms including 47 S. aureus strains, the tested S. aureus strains formed blue-colored colonies on the SkSA and all the other microbes failed to grow. The SkSA was then compared with Baird-Parker agar (BP) according to ISO 6888-1, Mannitol salt agar with egg yolk (MSEY), and 3M Petrifilm~(TM) STX (3M-STX) in 100 artificially contami nated food samples. The correlation coefficients between SkSA and BP, SkSA and MSEY, and SkSA and 3M-STX were 0.971, 0.989 and 0.996, respectively. Our results demonstrated that SkSA is a suitable alternative for the enumeration of S. aureus in foods.
机译:评价了用于新型金黄色葡萄球菌(SkSA)的Sanita-kunTM SA,一种新型的干片定量培养系统。用包括47 S在内的121种微生物评估了SkSA的包容性和排他性。金黄色葡萄球菌菌株,经测试的i。金黄色葡萄球菌菌株在SkSA上形成蓝色菌落,所有其他微生物均无法生长。然后在100个人工污染的食品样品中,根据ISO 6888-1将SkSA与Baird-Parker琼脂(BP),甘露醇盐蛋黄琼脂(MSEY)和3M PetrifilmTM STX(3M-STX)进行比较。 。 SkSA和BP,SkSA和MSEY以及SkSA和3M-STX之间的相关系数分别为0.971、0.989和0.996。我们的结果表明,SkSA是 S枚举的合适替代方法。食品中的金黄色葡萄球菌。

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