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Isolation and Characterization of a Purple Non-Sulfur Photosynthetic Bacterium Rhodopseudomonas faecalis Strain A from Swine Sewage Wastewater

机译:猪污水废水中紫色非硫光合细菌 Rhodopseudomonas faecalis 菌株A的分离与鉴定

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A purple non-sulfur photosynthetic bacterium (PNSB), PSB Strain A was isolated from swine sewage wastewater. Phylogenetic analysis revealed that PSB Strain A was most closely related to Rhodopseudomonas faecalis . Growth of the isolate under anaerobic-light conditions with a variety of carbon sources was investigated. Both PSB Strain A and the standard strain showed good growth with acetic acid, propionic acid, and n-butyric acid at a concentration of 20 mM. At the high concentration of 200 mM, PSB Strain A showed better growth in pyruvate, acetate, propionate, succinate and malate. By applying PSB Strain A to treat swine sewage wastewater, the concentration of VFAs, which were acetic acid and propionic acid, decreased from 158.0 mM to 120.2±2.9 mM, and 14.9 mM to 9.3±0.9 mM, respectively, after 216-h incubation. After 330-h incubation, the concentrations of TOC and ammonia nitrogen dropped from 4508.0 mg/L to 3104.0±451.5 mg/L, and 629.7 mg/L to 424.1±7.4 mg/L, respectively. The isolated PSB Strain A showed almost the same efficiency compared with the standard strain on the removal of VFAs and TOC. The results suggest the possibility of using the isolated strain to treat swine sewage wastewater.
机译:从猪污水中分离出紫色的非硫光合细菌(PSSB A)。系统发育分析表明,PSB菌株A与粪大红假单胞菌(Rhodopseudomonas faecalis)关系最密切。研究了在有多种碳源的厌氧光照条件下分离株的生长。 PSB菌株A和标准菌株在浓度为20 mM的乙酸,丙酸和正丁酸下均表现出良好的生长。在200 mM的高浓度下,PSB菌株A在丙酮酸盐,乙酸盐,丙酸盐,琥珀酸盐和苹果酸盐中表现出更好的生长。通过应用PSB菌株A处理猪污水,经过216小时的培养,乙酸和丙酸中的VFA浓度分别从158.0 mM降至120.2±2.9 mM,从14.9 mM降至9.3±0.9 mM。 。孵育330小时后,TOC和氨氮的浓度分别从4508.0 mg / L降至3104.0±451.5 mg / L和629.7 mg / L降至424.1±7.4 mg / L。分离的PSB菌株A在去除VFA和TOC方面显示出与标准菌株几乎相同的效率。结果表明使用分离的菌株处理猪污水的可能性。

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