• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biocontrol Science >Inter-Laboratory Study to Validate New Rapid Screening Methods for Kudoa septempunctata
【24h】

Inter-Laboratory Study to Validate New Rapid Screening Methods for Kudoa septempunctata

机译：实验室间研究，以验证 七叶锦葵 的新快速筛选方法

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Kudoa septempunctata is the causative agent of a food-borne disease associated with the ingestion of raw olive flounder. As the current qRT-PCR method for its detection is time-consuming, a rapid and simple method is required. Recently, a new real-time loop-mediated isothermal amplification (LAMP) method and an immunochromatography method, whose sensitivities are intended to be compatible with that designated in the official analytical method (10~(5) spores/g olive flounder), have been developed. To validate these new methods, we performed an inter-laboratory study across seven laboratories. Both methods could not detect less than 10~(4) spores/g; however, these methods were able to detect more than 10~(5) spores/g in olive flounder samples. These results demonstrated that the sensitivities of these methods were compatible with the designated level in the official analytical method. We concluded that these new methods were acceptable as the screening methods for K. septempunctata .
机译：锦葵（Kudoa septempunctata）是食源性疾病的诱因，与食用生的比目鱼有关。由于当前的qRT-PCR检测方法非常耗时，因此需要一种快速而简单的方法。最近，一种新的实时环介导的等温扩增（LAMP）方法和一种免疫色谱方法，其灵敏度与官方分析方法中指定的灵敏度（10〜（5）孢子/克比目鱼）兼容。已开发。为了验证这些新方法，我们在七个实验室之间进行了实验室间研究。两种方法都不能检测到低于10〜（4）孢子/ g;然而，这些方法能够检测出比目鱼样品中超过10〜（5）/ g的孢子。这些结果表明，这些方法的灵敏度与官方分析方法中指定的水平兼容。我们得出的结论是，这些新方法可以作为 K的筛选方法。 septempunctata。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号